<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>There is an optimized, validated and implemented method for determination of kaempferol through liquid chromatography in terms of isocratic separation in the proportion: 0.05% phosphoric acid and acetonitril (50:50) v/v. The limits of detection (LOD) and quantification (LOQ) were determined. The content of kaempferol in samples from <italic>Lilium candidum</italic> L. with the use of different concentrations of extract agent and after releasing kaempferol from dermal semisolid medicaments was investigated. The samples were prepared by various producers. The results obtained proved that the implemented method shows good validated parameters and is favourable for determination of kaempferol in samples from <italic>Lilium candidum</italic> L. It was kaempferol that evaluated best from the samples that were prepared by extraction of 80% ethanol. The contents in the samples were determined using the method, after releasing kaempferol from dermal semisolid medicaments.</p></abstract>

There is an optimized, validated and implemented method for determination of kaempferol through liquid chromatography in terms of isocratic separation in the proportion: 0.05% phosphoric acid and acetonitril (50:50) v/v. The limits of detection (LOD) and quantification (LOQ) were determined. The content of kaempferol in samples from Lilium candidum L. with the use of different concentrations of extract agent and after releasing kaempferol from dermal semisolid medicaments was investigated. The samples were prepared by various producers. The results obtained proved that the implemented method shows good validated parameters and is favourable for determination of kaempferol in samples from Lilium candidum L. It was kaempferol that evaluated best from the samples that were prepared by extraction of 80% ethanol. The contents in the samples were determined using the method, after releasing kaempferol from dermal semisolid medicaments.

Determination of kaempferol in extracts from <italic>Lilium candidum</italic> L. Liliaceae by means of liquid chromatography

Faculty of Pharmacy, Toxicological and Antidoping centre, Comenius University in Bratislava

Faculty of Pharmacy, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Comenius University in Bratislava

Faculty of Pharmacy, Department of Galenic Pharmacy, Comenius University in Bratislava

Faculty of Pharmacy, Department of Pharmacognosy and Botany, Comenius University in Bratislava

European Pharmaceutical Journal

Determination of kaempferol in extracts from  L. Liliaceae by means of liquid chromatography

There is an optimized, validated and implemented method for determination of kaempferol through liquid chromatography in terms of isocratic separation in the...

Determination of kaempferol in extracts from Lilium candidum L. Liliaceae by means of liquid chromatography

Published Online: Aug 13, 2012

Page range: 14 - 21

DOI: https://doi.org/10.2478/v10219-012-0015-7

Keywords
kaempferol, Lilium, isocratic separation, liquid chromatography

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