<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the study was to develop a rapid, simple, effective and reproducible TLC method for separation of a naturally occurring mixture of ursolic and oleanolic acids. Because of the similarity of chemical structures, <italic>in situ</italic> derivatisation by iodine was necessary to separate these triterpenic acids. Separation was achieved on silica gel plates. After derivatisation, a chromatographic plate was developed with the mobile phase consisting of light petrol, ethyl acetate and acetone (8.2:1.8:0.1, v/v/v) following visualisation by spraying with sulphuric acid in diethylether (25%, v/v) and heating to 120°C for 5 min. The method used enabled chromatographical differentiation of ursolic and oleanolic acid mixtures in all tested mutual ratios.</p></abstract>

The aim of the study was to develop a rapid, simple, effective and reproducible TLC method for separation of a naturally occurring mixture of ursolic and oleanolic acids. Because of the similarity of chemical structures, in situ derivatisation by iodine was necessary to separate these triterpenic acids. Separation was achieved on silica gel plates. After derivatisation, a chromatographic plate was developed with the mobile phase consisting of light petrol, ethyl acetate and acetone (8.2:1.8:0.1, v/v/v) following visualisation by spraying with sulphuric acid in diethylether (25%, v/v) and heating to 120°C for 5 min. The method used enabled chromatographical differentiation of ursolic and oleanolic acid mixtures in all tested mutual ratios.

Contribution to the TLC separation of ursolic and oleanolic acid mixture

Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic

European Pharmaceutical Journal

{"article-title":"Contribution to the TLC separation of ursolic and oleanolic acid mixture"}

The aim of the study was to develop a rapid, simple, effective and reproducible TLC method for separation of a naturally occurring mixture of ursolic and...

Contribution to the TLC separation of ursolic and oleanolic acid mixture

Published Online: Nov 25, 2011

Page range: 56 - 61

DOI: https://doi.org/10.2478/v10219-011-0006-0

Keywords
oleanolic acid, ursolic acid, TLC, separation

This content is open access.

Contribution to the TLC separation of ursolic and oleanolic acid mixture

Published Online: Nov 25, 2011

Page range: 56 - 61

DOI: https://doi.org/10.2478/v10219-011-0006-0

Keywordsoleanolic acid, ursolic acid, TLC, separation

This content is open access.

Keywords
oleanolic acid, ursolic acid, TLC, separation