<abstract xmlns="http://www.w3.org/1999/xhtml">

 The aim of the work was to find out what is the prevalence and burden of <italic>Campylobacter </italic>sp. in the poultry exudates, what are the predominant species, how diverse they are, and can poultry exudates be considered as the essential carriers of campylobacters posing a health risk of campylobacteriosis in Poland. Broilers’ and turkeys’ exudates, collected directly from the bulk containers at the day of delivery were subjected to pre-enrichment and direct plate counting according to ISO 10272 (1995) with presumptive <italic>C.</italic><italic>jejuni </italic>strains subjected to confirmatory genetic identification by the nested PCR and RAPD typing. The results indicated that over 90% of the exudates tested carried <italic>Campylobacter </italic>sp., with their number being significantly higher in broilers’ (103 to105 cfu mL-1) compared to turkeys’ (102 to 104 cfu mL-1) (P≤0.05), and essentially higher (P≤0.05) for the broiler parts’ “skin on” exudates. <italic>C.</italic><italic>jejuni </italic>was the only species present in the turkeys’ and predominant in broilers’ parts’ exudates. RAPD-typing of the isolates revealed 14 distinct RAPD types with the majority of <italic>C. jejuni </italic>isolates considered to be closely related.
</abstract>

The aim of the work was to find out what is the prevalence and burden of Campylobacter sp. in the poultry exudates, what are the predominant species, how diverse they are, and can poultry exudates be considered as the essential carriers of campylobacters posing a health risk of campylobacteriosis in Poland. Broilers’ and turkeys’ exudates, collected directly from the bulk containers at the day of delivery were subjected to pre-enrichment and direct plate counting according to ISO 10272 (1995) with presumptive C.jejuni strains subjected to confirmatory genetic identification by the nested PCR and RAPD typing. The results indicated that over 90% of the exudates tested carried Campylobacter sp., with their number being significantly higher in broilers’ (103 to105 cfu mL-1) compared to turkeys’ (102 to 104 cfu mL-1) (P≤0.05), and essentially higher (P≤0.05) for the broiler parts’ “skin on” exudates. C.jejuni was the only species present in the turkeys’ and predominant in broilers’ parts’ exudates. RAPD-typing of the isolates revealed 14 distinct RAPD types with the majority of C. jejuni isolates considered to be closely related.

Is Poultry Exudate a Missing Link in Spreading Campylobacter SP. In Poland?

Department of Applied Microbiology and Biotechnology

West Pomeranian University of Technology in Szczecin

Journal of Veterinary Research

{"article-title":"Is Poultry Exudate a Missing Link in Spreading Campylobacter SP. In Poland?"}

The aim of the work was to find out what is the prevalence and burden of Campylobacter sp. in the poultry exudates, what are the predominant species, how...

Is Poultry Exudate a Missing Link in Spreading Campylobacter SP. In Poland?

Published Online: Jan 17, 2013

Page range: 499 - 505

DOI: https://doi.org/10.2478/v10213-012-0088-z

Keywords
Campylobacter sp., poultry exudates, bulk containers, RAPD-types

This content is open access.

Is Poultry Exudate a Missing Link in Spreading Campylobacter SP. In Poland?

Published Online: Jan 17, 2013

Page range: 499 - 505

DOI: https://doi.org/10.2478/v10213-012-0088-z

KeywordsCampylobacter sp., poultry exudates, bulk containers, RAPD-types

This content is open access.

Keywords
Campylobacter sp., poultry exudates, bulk containers, RAPD-types