<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>In the study, the genetic similarity between <italic>Dasypyrum villosum</italic> L. (P.) Candargy and <italic>Triticum</italic> L. and <italic>Secale</italic> L. species was studied on the basis of ISSR markers. As a very polymorphic, effective and reproducible method, ISSR can be successfully employed to evaluate polymorphism between and inside different species. The polymorphic information content values (PIC) of ISSR method ranged from 0.57 to 0.87, with the mean value of 0.7. The genetic similarity of the forms analyzed ranged from 0.27 to 0.97, with the mean value of 0.47, indicating their high diversity. A higher similarity of <italic>Dasypyrum villosum</italic> to <italic>Triticum</italic> species, in comparison with <italic>Secale</italic> was found - the mean Dice genetic similarity index between genera was calculated at 0.40 for <italic>Dasypyrum</italic> and <italic>Triticum</italic>, and at 0.31 for <italic>Dasypyrum</italic> and <italic>Secale</italic>.</p></abstract>

In the study, the genetic similarity between Dasypyrum villosum L. (P.) Candargy and Triticum L. and Secale L. species was studied on the basis of ISSR markers. As a very polymorphic, effective and reproducible method, ISSR can be successfully employed to evaluate polymorphism between and inside different species. The polymorphic information content values (PIC) of ISSR method ranged from 0.57 to 0.87, with the mean value of 0.7. The genetic similarity of the forms analyzed ranged from 0.27 to 0.97, with the mean value of 0.47, indicating their high diversity. A higher similarity of Dasypyrum villosum to Triticum species, in comparison with Secale was found - the mean Dice genetic similarity index between genera was calculated at 0.40 for Dasypyrum and Triticum, and at 0.31 for Dasypyrum and Secale.

Application of the ISSR method to estimate the genetic similarity of <italic>Dasypyrum villosum</italic> (L.) P. Candargy Greek populations to <italic>Triticum</italic> and <italic>Secale</italic> species

Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland

Biodiversity Research and Conservation

Application of the ISSR method to estimate the genetic similarity of  (L.) P. Candargy Greek populations to  and  species

In the study, the genetic similarity between Dasypyrum villosum L. (P.) Candargy and Triticum L. and Secale L. species was studied on the basis of ISSR...

Application of the ISSR method to estimate the genetic similarity of Dasypyrum villosum (L.) P. Candargy Greek populations to Triticum and Secale species

Published Online: Oct 20, 2011

Page range: 7 - 12

DOI: https://doi.org/10.2478/v10119-011-0002-1

Keywords
ISSR, molecular markers, genetic similarity

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