1. bookVolume 2 (2009): Issue 1 (March 2009)
Journal Details
License
Format
Journal
eISSN
1337-9569
ISSN
1337-6853
First Published
19 Jun 2009
Publication timeframe
4 times per year
Languages
English
access type Open Access

Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency

Journal Details
License
Format
Journal
eISSN
1337-9569
ISSN
1337-6853
First Published
19 Jun 2009
Publication timeframe
4 times per year
Languages
English
Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency

Authors evaluated the incidence of total chromosomal aberrations (CA) and their types - chromatid-type (CTA) and chromosome-type (CSA) in peripheral blood lymphocytes from 72 oncologic unit's workers occupationally exposed to cytostatics in relationship to polymorphisms of DNA repair genes XPD, XPG and XPC. The cytogenetic analysis was used for determination of chromosomal aberrations frequency and PCR-RFLP method for polymorphisms of genes. Statistically higher frequency of total CA was detected in exposed group as compared to control (1.90±1.34% vs. 1.26±0.93%; Mann-Whitney U-test, p=0.001). There was not detected any difference between CTA and CSA (0.92±1.04% vs. 0.98±1.17%). Similarly, in genes XPD exon 23 and XPC exon 15 wasn't detected any difference neither in total chromosomal aberrations nor in CTA and CSA types. Statistically significant decrease of total chromosomal aberrations and CTA-type with presence of variant allele C was detected in gene XPG exon 15. Authors pointed out the importance of individual susceptibility factors in evaluation of effects of genotoxic agents, in that event, when the concentration does not meet the occupational exposure limit.

Keywords

AHEM. (2007). Metody biologického monitorování genotoxických účinků faktorů prostředí. Cytogenetická analýza periferních lymfocytů. Aktualizácia platnej štandardnej metodiky. AHEM 1/2007, SZÚ, Praha, 30 pp.Search in Google Scholar

Angelini S, Kumar R, Carbone F, Bermejo JL, Maffei F, Cantelli-Forti G, Hemminki K and Hrelia P. (2008). Inherited susceptibility to bleomycin-induced micronuclei: correlating polymorphisms in GSTT1, GSTM1 and DNA repair genes with mutagen sensitivity. Mutat Res 638: 90-97.Search in Google Scholar

Burgaz S, Karahalil B, Canhi Z, Terzioglu F, Ancel G, Anzion RB, Bos RP and Hüttner E. (2002). Assessment of genotoxic damage in nurses occupationally exposed to antineoplastics by the analysis of chromosomal aberrations. Hum Exp Toxicol 21: 129-135.Search in Google Scholar

Cavallo D, Ursini CL, Omodeo-Sale E and Iavicoli S. (2007). Micronucleus induction and FISH analysis in buccal cells and lymphocytes of nurses administering antineoplastic drugs. Mutat Res 628: 11-18.Search in Google Scholar

Cavallo D, Ursini LC, Perniconi B, Di Francesco A, Giglio M, Rubino FM, Marinaccio A and Iavicoli S. (2005). Evaluation of genotoxic effects induced exposure to antineoplastic drugs in lymphocytes and exfoliated buccal cells of oncology nurses and pharmacy employees. Mutat Res 587: 45-51.Search in Google Scholar

Connor TH. (2006). Hazardous anticancer drugs in health care: environmental exposure assessment. Ann N Acad Sci 1076: 615-623.Search in Google Scholar

Deng H, Hang M, He J, Wu W, Jin L, Zheng W, Lou J and Wang B. (2005). Investigating genetic damage in workers occupationally exposed to methotrexate using three genetic end-points. Mutagenesis 20: 351-357.Search in Google Scholar

Fransman W, Huizer D, Tuerk J and Kromhout H. (2007a). Inhalation and dermal exposure to eight antineoplastic drugs in an industrial laundry facility. Int Arch Occup Environ Health 80: 396-403.10.1007/s00420-006-0148-x17021843Search in Google Scholar

Fransman W, Roeleveld N, Peelen S, de Kort W, Kromhout H and Heederik D. (2007b). Nurses with dermal exposure to antineoplastic drugs: reproductive outcomes. Epidemiology 18: 112-119.10.1097/01.ede.0000246827.44093.c117099323Search in Google Scholar

Fransman W, Vermeulen R and Kromhout H. (2005). Dermal exposure to cyclo-phosphamide in hospitals during preparation, nursing and cleaning activities. Int Arch Occup Environ Health 78: 403-412.Search in Google Scholar

Hemminki K. (2001). Biomarkers of exposure and effect for carcinogenicity. Appendix I. In: WHO Library Cataloguing-in-Publication Data, Biomarkers in risk assessment: validity and validation (Environmental health criteria) 222, WHO, Geneva, 241pp, 47-94.Search in Google Scholar

Hou SM, Fält S, Angelini S, Yang K, Nyberg F, Lambert B and Hemminki K. (2002). The XPD variant alleles are associated with increased aromatic DNA adduct level and lung cancer risk. Carcinogenesis 23: 599-603.Search in Google Scholar

Kumar R, Höglund L, Zhao CH, Försti A, Snellman E and Hemminki K. (2003). Single nucleotide polymorphisms in the XPG gene: determination of role in DNA repair and breast cancer risk. Int J Cancer 103: 671-675.Search in Google Scholar

Laczmanska I, Gil J, Karpinski P, Stembalska A, Trusewicz A, Pesz K, Ramsey D, Schlade-Bartusiak K, Blin N and Sasiadek MM. (2007). Polymorphism in nucleotide excision repair gene XPC correlates with bleomycin-induced chromosomal aberrations. Environ Mol Mutagen 48: 666-671.Search in Google Scholar

Major J, Jakab MG and Tompa A. (1999). The frequency of induced premature centromere division in human populations occupationally exposed to genotoxic. Mutat Res 445: 241-249.Search in Google Scholar

Maluf SW and Erdtmann B. (2000). Follow-up study of the genetic damage in lymphocytes of pharmacists and nurses handling antineoplastic drugs evaluated by cytokinesis-block micronuclei analysis and single cell gel electrophoresis. Mutat Res 471: 21-27.Search in Google Scholar

Mohrenweiser HW, Xi T, Vazquez-Matias J and Jones IM. (2002). Identification of 127 amino acid substitution variants in screening 37 DNA repair genes in humans. Cancer Epidemiol Biomarkers Prev 11: 1054-1064.Search in Google Scholar

Musak L, Vodicka P, Klimentová G, Soucek P, Hánová M, Mikulková R, Buchancová J, Vodicková L, Poláková V and Péc M. (2006). Chromosomal damage and polymorphisms of DNA repair genes XRCC1 and XRCC3 in workers exposed to cytostatics. Neuro Endocrinol Lett 27 (Suppl 2): 57-60.Search in Google Scholar

Norppa H. (2004). Cytogenetic biomarkers and genetic polymorphisms. Toxicol Lett 149: 309-334.Search in Google Scholar

Norppa H. (2003). Genetic susceptibility, biomarker responses, and cancer. Mutat Res 544: 339-348.Search in Google Scholar

Pilger A, Köhler I, Stettner H, Mader RM, Rizovski B, Terkola R, Diem E, Franz-Hainzl E, Konnaris CM Valic E and Rüdinger HW. (2000). Long-term monitoring of sister chromatid exchanges and micronucleus frequencies in pharmacy personnel occupationally exposed to cytostatic drugs. Int Arch Occup Environ Health 73: 442-448.Search in Google Scholar

Rekhadevi PV, Sailaja N, Chandrasekhar M, Mahboob M, Rahman MF and Grover P. (2007). Genotoxicity assessment in oncology nurses handling anti-neoplastic drugs. Mutagenesis 22: 395-401.Search in Google Scholar

Rombaldi F, Cassini C, Salvator M, Saffi J and Erdtmann B. (2009). Occupational risk assessment of genotoxicity and oxidative stress in workers handling antineoplastic drugs during a working week. Mutagenesis 24: 143-148.Search in Google Scholar

Rubes J, Kucharová S, Vozdová M, Musilová P and Zudová Z. (1998). Cytogenetic analysis of peripheral lymphocytes in medical personnel by means of FISH. Mutat Res 412: 293-298.Search in Google Scholar

Skjelbred CF, Svendsen M, Haugan V, Eek AK, Clausen KO, Svendsen MV and Hansteen IL. (2006). Influence of DNA repair gene polymorphisms of hOGG1, XRCC1, XRCC3, ERCC2 and the folate metabolism gene MTHFR on chromosomal aberration frequencies. Mutat Res 602: 151-162.Search in Google Scholar

Sudprasert W, Navasumrit P and Ruchirawat M. (2006). Effects of low-dose gamma radiation on DNA damage, chromosomal aberration and expression of repair genes in human blood cells. Int J Hyg Environ Health 209: 503-511.Search in Google Scholar

Testa A, Giachelia M, Palma S, Appolloni M, Padua L, Tranfo G, Spagnoli M, Tirindelli D and Cozzi R. (2007). Occupational exposure to antineoplastic agents induces a high level of chromosome damage. Lack of an effect of GST polymorphisms. Toxicol Appl Pharmacol 223: 46-55.Search in Google Scholar

Tuimala J, Szekely G, Gundy S, Hirvonen A and Norppa H. (2002). Genetic polymorphisms of DNA repair and xenobiotic-metabolizing enzymes: role in mutagen sensitivity. Carcinogenesis 23: 1003-1008.Search in Google Scholar

Tuimala J, Szekely G, Wikman H, Järventaus H, Hirvonen A, Gundy S and Norppa H. (2004). Genetic polymorphisms of DNA repair and xenobiotic-metabolizing enzymes: effects on levels of sister chromatid exchanges and chromosomal aberrations. Mutat Res 554: 319-333. Erratum in Mutat Res 2005, 570: 303.Search in Google Scholar

Vodicka P, Kumar R, Stetina R, Sanyal S, Soucek P, Haufroid V, Dusinska M, Kuricova M, Zamecnikova M, Musak L, Buchancova J, Norppa H, Hirvonen A, Vodickova L, Naccarati A, Matousu Z and Hemminki K. (2004). Genetic polymorphisms in DNA repair genes and possible links with DNA repair rates, chromosomal aberrations and single-strand breaks in DNA. Carcinogenesis 25: 757-763.Search in Google Scholar

Vorlíček J, Vyzula R and Adam Z. (2000). Praktická onkologie, vybrané kapitoly. Grada Publishing s.r.o., Praha, pp.77-154.Search in Google Scholar

Wick U and Gebhart E. (2005). Studies on the action of mitomycin C and bleomycin on telomere lengths of human lymphocyte chromosomes. Int J Mol Med 16: 463-469.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo