<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Prior to the registration of a new variety, it is required to undergo Distinctness, Uniformity and Stability (DUS) testing. Preparing a newly developed variety to meet the requirements of DUS testing is a lengthy process, particularly regarding aspects of uniformity and stability. Field testing of a large number of lines is time and resource intensive. In addition, the expression of certain traits may be influenced by environmental conditions. The use of DNA markers may allow rapid assessment of the level of genetic diversity within a particular line or variety, and to remove individuals that are genetically differentiated, thus accelerating the homogenisation of a newly developed variety. In this study, we utilised AFLP and the iPBS marker techniques to assess genetic variation within advanced breeding lines of several cereal species (triticale, wheat, barley). The combined use of molecular and morphological selection over three years of analysis and selection resulted in the reduction of genetic diversity within breeding lines.</p></abstract>

Prior to the registration of a new variety, it is required to undergo Distinctness, Uniformity and Stability (DUS) testing. Preparing a newly developed variety to meet the requirements of DUS testing is a lengthy process, particularly regarding aspects of uniformity and stability. Field testing of a large number of lines is time and resource intensive. In addition, the expression of certain traits may be influenced by environmental conditions. The use of DNA markers may allow rapid assessment of the level of genetic diversity within a particular line or variety, and to remove individuals that are genetically differentiated, thus accelerating the homogenisation of a newly developed variety. In this study, we utilised AFLP and the iPBS marker techniques to assess genetic variation within advanced breeding lines of several cereal species (triticale, wheat, barley). The combined use of molecular and morphological selection over three years of analysis and selection resulted in the reduction of genetic diversity within breeding lines.

Use of DNA markers for cereal line uniformity assessment

Genetic Resource Centre, LSFRI "Silava", Rīgas iela 111, Salaspils, LV-2169, LATVIA

State Stende Cereal Breeding Institute, "Dižzemes", Dižstende, Lībagu pag., Talsu nov., LV-3258, LATVIA

Priekuļi Plant Breeding Institute, Zinātnes iela 1a, Priekuļi, LV-4126, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

{"article-title":"Use of DNA markers for cereal line uniformity assessment"}

Prior to the registration of a new variety, it is required to undergo Distinctness, Uniformity and Stability (DUS) testing. Preparing a newly developed variety...

Use of DNA markers for cereal line uniformity assessment

Published Online: Jun 28, 2012

Page range: 21 - 25

DOI: https://doi.org/10.2478/v10046-011-0041-1

Keywords
DUS testing, genetic uniformity, DNA markers, AFLP, iPBS

This content is open access.

Use of DNA markers for cereal line uniformity assessment

Published Online: Jun 28, 2012

Page range: 21 - 25

DOI: https://doi.org/10.2478/v10046-011-0041-1

KeywordsDUS testing, genetic uniformity, DNA markers, AFLP, iPBS

This content is open access.

Keywords
DUS testing, genetic uniformity, DNA markers, AFLP, iPBS