<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p><italic>Screening of celery cultivars with random amplified primers is a way of avoiding mistakes in distinguishing similar genotypes. Six primers showed distinct polymorphism between the studied cultivars. The number of bands varied from 7 to 13. Suitable primers generated 52 markers of which 22 were polymorphic. A similarity matrix was created using Jaccard's coefficient. The group average method was employed to construct a dendrogram. Based on RAPD marker profiles the cultivars were grouped into three clusters coinciding with the cultivated types, var. dulce (salad celery), and var. rapaceum (turnip celery), var. secalinum (cutting celery). The salad celery entries were similar to plants of turnip celery with similarity 0.73, and the distance between genotypes of cutting celery and turnip celery was 0.68. Although only 12 cultivars have been analysed, the specific product amplified with OPX1 was observed in the three studied cultivars of cutting celery</italic>.</p></abstract>

Screening of celery cultivars with random amplified primers is a way of avoiding mistakes in distinguishing similar genotypes. Six primers showed distinct polymorphism between the studied cultivars. The number of bands varied from 7 to 13. Suitable primers generated 52 markers of which 22 were polymorphic. A similarity matrix was created using Jaccard's coefficient. The group average method was employed to construct a dendrogram. Based on RAPD marker profiles the cultivars were grouped into three clusters coinciding with the cultivated types, var. dulce (salad celery), and var. rapaceum (turnip celery), var. secalinum (cutting celery). The salad celery entries were similar to plants of turnip celery with similarity 0.73, and the distance between genotypes of cutting celery and turnip celery was 0.68. Although only 12 cultivars have been analysed, the specific product amplified with OPX1 was observed in the three studied cultivars of cutting celery.

Discrimination Between Celery Cultivars with the Use of Rapd Markers

Russian Research Institute of Vegetable Breeding and Seed Production, p/o Lesnoy Gorodok, Odintsovskij region, Moskovskaya oblast, 143080, RUSSIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

{"article-title":"Discrimination Between Celery Cultivars with the Use of Rapd Markers"}

Screening of celery cultivars with random amplified primers is a way of avoiding mistakes in distinguishing similar genotypes. Six primers showed distinct...

Discrimination Between Celery Cultivars with the Use of Rapd Markers

Published Online: Apr 02, 2009

Page range: 219 - 222

DOI: https://doi.org/10.2478/v10046-009-0003-z

Keywords
Apium graveolens, Randomly Amplified Polymorphic DNA, molecular markers

This content is open access.

Discrimination Between Celery Cultivars with the Use of Rapd Markers

Published Online: Apr 02, 2009

Page range: 219 - 222

DOI: https://doi.org/10.2478/v10046-009-0003-z

KeywordsApium graveolens, Randomly Amplified Polymorphic DNA, molecular markers

This content is open access.

Keywords
Apium graveolens, Randomly Amplified Polymorphic DNA, molecular markers