<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The RP-TLC method was used to determine the dissociation constant of chrysin (5,7-dihydroxyflavone) in methanol-aqueous (1:1 v/v) solutions. In this method the <italic>pK</italic> value was quantified on the basis of retention data and a retention model. The accuracy of determining the model parameters was analysed using the following statistical criteria: the sum of the squared differences between the experimental and theoretical data, approximation of standard deviation, and the Fisher test. Besides, in this work the potentiometric method was used. Investigations were carried out at ionic strength I=0.1 at T= 298 K. The dissociation constant were determined using the Rossotti method as well as the numerical method based on the procedures of non-linear curve fitting using Microsoft Excel Solver and the user-defined function. It has been found that the differences in the evaluated <italic>pK</italic> values were relatively small and did not exceed 1.2%.</p></abstract>

The RP-TLC method was used to determine the dissociation constant of chrysin (5,7-dihydroxyflavone) in methanol-aqueous (1:1 v/v) solutions. In this method the pK value was quantified on the basis of retention data and a retention model. The accuracy of determining the model parameters was analysed using the following statistical criteria: the sum of the squared differences between the experimental and theoretical data, approximation of standard deviation, and the Fisher test. Besides, in this work the potentiometric method was used. Investigations were carried out at ionic strength I=0.1 at T= 298 K. The dissociation constant were determined using the Rossotti method as well as the numerical method based on the procedures of non-linear curve fitting using Microsoft Excel Solver and the user-defined function. It has been found that the differences in the evaluated pK values were relatively small and did not exceed 1.2%.

Investigations of selected properties of pharmacologically active compounds by chromatographic and potentiometric methods on the chrysin example

Department of Inorganic and Analytical Chemistry

Department of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland

Polish Journal of Chemical Technology

The RP-TLC method was used to determine the dissociation constant of chrysin (5,7-dihydroxyflavone) in methanol-aqueous (1:1 v/v) solutions. In this method the...

Investigations of selected properties of pharmacologically active compounds by chromatographic and potentiometric methods on the chrysin example

Published Online: Apr 03, 2008

Page range: 41 - 44

DOI: https://doi.org/10.2478/v10026-008-0010-x

Keywords
RP-HPLC, retention model, chrysin, dissociation constant

This content is open access.

Investigations of selected properties of pharmacologically active compounds by chromatographic and potentiometric methods on the chrysin example

Published Online: Apr 03, 2008

Page range: 41 - 44

DOI: https://doi.org/10.2478/v10026-008-0010-x

KeywordsRP-HPLC, retention model, chrysin, dissociation constant

This content is open access.

Keywords
RP-HPLC, retention model, chrysin, dissociation constant