<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><bold>Background.</bold> Gastric cancer is one of the leading malignancies with a poor prognosis and low survival rates. Although the mechanisms underlying its development are still unknown, there is a consensus that genetic instability, inactivation of tumor suppressor genes and over-expression of oncogenes are involved in the early and late stages of gastric carcinogenesis. In the present study we wanted to display differential expression of seven oncogenes, namely CCNE1, EGF, ERBB3, FGF4, HRG1, HGFR and TDGF1.<bold>Patients and methods.</bold> We employed a method based on the multiplex reverse transcription polymerase chain (RT-PCR) method with a fluorescence detection.<bold>Results.</bold> More than half of patients (74.3%) out of total 74 with gastric adenocarcinoma had over-expressed at least one oncogene, with the exception of FGF4, which was expressed in tumor tissue of less than one third of patients. 56.8% of the patients patients showed over-expression of two or more oncogenes.<bold>Conclusions.</bold> Patients with precancerous lesions had elevated levels of TDGF1 or cripto-1 (64.9%) and CCNE1 (57.1%), suggesting that they could be used as markers for an early detection of malignant changes in stomach. Finally, the fluorescent multiplex RT-PCR method could be of value for rapid assessment of oncogene mRNA levels in small samples of tumor or precancerous biopsies.</abstract>

Background. Gastric cancer is one of the leading malignancies with a poor prognosis and low survival rates. Although the mechanisms underlying its development are still unknown, there is a consensus that genetic instability, inactivation of tumor suppressor genes and over-expression of oncogenes are involved in the early and late stages of gastric carcinogenesis. In the present study we wanted to display differential expression of seven oncogenes, namely CCNE1, EGF, ERBB3, FGF4, HRG1, HGFR and TDGF1.Patients and methods. We employed a method based on the multiplex reverse transcription polymerase chain (RT-PCR) method with a fluorescence detection.Results. More than half of patients (74.3%) out of total 74 with gastric adenocarcinoma had over-expressed at least one oncogene, with the exception of FGF4, which was expressed in tumor tissue of less than one third of patients. 56.8% of the patients patients showed over-expression of two or more oncogenes.Conclusions. Patients with precancerous lesions had elevated levels of TDGF1 or cripto-1 (64.9%) and CCNE1 (57.1%), suggesting that they could be used as markers for an early detection of malignant changes in stomach. Finally, the fluorescent multiplex RT-PCR method could be of value for rapid assessment of oncogene mRNA levels in small samples of tumor or precancerous biopsies.

Assessment of differential expression of oncogenes in adenocarcinoma of stomach with fluorescent labeling and simultaneous amplification of gene transcripts

Medical Center for Molecular Biology, Faculty of Medicine, Institute of Biochemistry, Ljubljana, Slovenia

Clinical Department for Anesthesiology and Intensive Care of Operative Branches, University Clinical Center, Ljubljana, Slovenia

Department of Animal Science, Biotechnical Faculty, Domzale, Slovenia

Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria

Clinical Department for Abdominal Surgery, University Clinical Center, Ljubljana, Slovenia

Instrumental Analysis and Bioanalysis, Saarland University, Im Stadtwald, 66123 Saarbruecken, Germany

Radiology and Oncology

Background. Gastric cancer is one of the leading malignancies with a poor prognosis and low survival rates. Although the mechanisms underlying its development...

Assessment of differential expression of oncogenes in adenocarcinoma of stomach with fluorescent labeling and simultaneous amplification of gene transcripts

Published Online: Jan 21, 2008

Page range: 174 - 182

DOI: https://doi.org/10.2478/v10019-007-0028-3

Keywords
stomach neoplasms - genetics, adenocarcinoma, reverse transcriptase polymerase chain reaction, oncogenes

This content is open access.

Assessment of differential expression of oncogenes in adenocarcinoma of stomach with fluorescent labeling and simultaneous amplification of gene transcripts

Published Online: Jan 21, 2008

Page range: 174 - 182

DOI: https://doi.org/10.2478/v10019-007-0028-3

Keywordsstomach neoplasms - genetics, adenocarcinoma, reverse transcriptase polymerase chain reaction, oncogenes

This content is open access.

Keywords
stomach neoplasms - genetics, adenocarcinoma, reverse transcriptase polymerase chain reaction, oncogenes