<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated. Saliva represents an alternative matrix for therapeutic monitoring of antiepileptic drugs due to the increasing interest in free drug concentration. The proposed method involved solid-phase extraction for sample preparation and yielded very good mean recoveries of 99.4 % and 97.9 % for valproic acid and IS, respectively. The calibration function for valproic acid was linear over the concentration range of 1.0-50.0 μg mL-1 (<italic>R</italic>2 = 0.9989). Within-run and between-run precision and accuracy were studied at four concentrations and RSDs were less than 7.3 and 2.2 %, while accuracy values were higher than 96.8 and 97.5 %, respectively. The described method provides sensitivity, linearity, precision, accuracy and is suitable for analyses of valproic acid in saliva samples.</abstract>

A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated. Saliva represents an alternative matrix for therapeutic monitoring of antiepileptic drugs due to the increasing interest in free drug concentration. The proposed method involved solid-phase extraction for sample preparation and yielded very good mean recoveries of 99.4 % and 97.9 % for valproic acid and IS, respectively. The calibration function for valproic acid was linear over the concentration range of 1.0-50.0 μg mL-1 (R2 = 0.9989). Within-run and between-run precision and accuracy were studied at four concentrations and RSDs were less than 7.3 and 2.2 %, while accuracy values were higher than 96.8 and 97.5 %, respectively. The described method provides sensitivity, linearity, precision, accuracy and is suitable for analyses of valproic acid in saliva samples.

Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva

Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University »Ss Cyril and Methodius«, Skopje, Macedonia

Department of Pharmacy, Medical Sciences Faculty, State University of Tetova, Tetovo, Macedonia

Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, University »Ss Cyril and Methodius«, Skopje, Macedonia

Clinic of Neurology, Faculty of Medicine, University »Ss Cyril and Methodius«, Skopje, Macedonia

Acta Pharmaceutica

A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated....

Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva

Published Online: Jun 28, 2012

Page range: 211 - 220

DOI: https://doi.org/10.2478/v10007-012-0015-0

Keywords
valproična kiselina, slina, ekstrakcija na čvrstoj fazi, visokotlačna tekućinska kromatografija s visokom učinkovitosti, validacija

This content is open access.

Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva

Published Online: Jun 28, 2012

Page range: 211 - 220

DOI: https://doi.org/10.2478/v10007-012-0015-0

Keywordsvalproična kiselina, slina, ekstrakcija na čvrstoj fazi, visokotlačna tekućinska kromatografija s visokom učinkovitosti, validacija

This content is open access.

Keywords
valproična kiselina, slina, ekstrakcija na čvrstoj fazi, visokotlačna tekućinska kromatografija s visokom učinkovitosti, validacija