<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L-1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L-1 HCl standard. During method's optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.</abstract>

A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L-1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L-1 HCl standard. During method's optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.

Simultaneous determination of iron and copper in children's sera by FAAS

Faculty of Pharmacy and Biochemistry Zagreb, University of Zagreb, Croatia

HALMED - Agency for Medicinal Products and Medical Devices, Zagreb, Croatia

Children's Hospital Srebrnjak, Zagreb, Croatia

Acta Pharmaceutica

{"article-title":"Simultaneous determination of iron and copper in children's sera by FAAS"}

A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is...

Simultaneous determination of iron and copper in children's sera by FAAS

Published Online: Mar 15, 2011

Page range: 93 - 102

DOI: https://doi.org/10.2478/v10007-011-008-4

Keywords
željezo, bakar, dječji serum, FAAS, određivanje u jednom koraku

This content is open access.

Simultaneous determination of iron and copper in children's sera by FAAS

Published Online: Mar 15, 2011

Page range: 93 - 102

DOI: https://doi.org/10.2478/v10007-011-008-4

Keywordsželjezo, bakar, dječji serum, FAAS, određivanje u jednom koraku

This content is open access.

Keywords
željezo, bakar, dječji serum, FAAS, određivanje u jednom koraku