<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Cisplatin-loaded protransfersome system was prepared and characterized for <italic>in vitro</italic> drug permeation, drug deposition and antitumor effect. A histopathological study and a genotoxicity study were also done. The skin permeation data of cisplatin from protransfersome gel formulation revealed 494.33 ± 11.87 μg cm<sup>-2</sup>, which was significantly higher than that from the control plain drug solution in 0.9 % NaCl (<italic>p</italic> &lt; 0.001). Untreated group of animals showed invasive moderately differentiated keratinizing squamous cell carcinoma (malignant stage). However, with cisplatin loaded protransfersome gel system simple epithelial hyperplasia (pre-cancerous stage) with no cancerous growth was observed. Also, a significant induction in micronucleus formation was found in the group that was treated with injectable intraperitoneal cisplatin preparation in 0.9 % saline as compared to the group treated with topical protransfersome gel formulation. The findings of this research work appear to support improved, site-specific and localized drug action in the skin, thus providing a better option for dealing with skin related problems like squamous cell carcinoma.</p></abstract>

Cisplatin-loaded protransfersome system was prepared and characterized for in vitro drug permeation, drug deposition and antitumor effect. A histopathological study and a genotoxicity study were also done. The skin permeation data of cisplatin from protransfersome gel formulation revealed 494.33 ± 11.87 μg cm-2, which was significantly higher than that from the control plain drug solution in 0.9 % NaCl (p < 0.001). Untreated group of animals showed invasive moderately differentiated keratinizing squamous cell carcinoma (malignant stage). However, with cisplatin loaded protransfersome gel system simple epithelial hyperplasia (pre-cancerous stage) with no cancerous growth was observed. Also, a significant induction in micronucleus formation was found in the group that was treated with injectable intraperitoneal cisplatin preparation in 0.9 % saline as compared to the group treated with topical protransfersome gel formulation. The findings of this research work appear to support improved, site-specific and localized drug action in the skin, thus providing a better option for dealing with skin related problems like squamous cell carcinoma.

Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinoma

School of Pharmaceutical Sciences, Rajiv Gandhi Technical University, Airport Bypass Road, Gandhi Nagar Bhopal (MP)-462036, India

Jawaharlal Nehru Cancer Hospital and Research Center, Post Box. No. 32 Idgah Hills, Bhopal-462001 (MP), India

Acta Pharmaceutica

Cisplatin-loaded protransfersome system was prepared and characterized for in vitro drug permeation, drug deposition and antitumor effect. A histopathological...

Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinoma

Published Online: Mar 15, 2011

Page range: 63 - 71

DOI: https://doi.org/10.2478/v10007-011-0004-8

Keywords
cisplatin, protransfersom, topička kemoterapija, oštećenje gena, stanice koštane srži, polikromatski eritrociti s mikronukleusom

This content is open access.

Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinoma

Published Online: Mar 15, 2011

Page range: 63 - 71

DOI: https://doi.org/10.2478/v10007-011-0004-8

Keywordscisplatin, protransfersom, topička kemoterapija, oštećenje gena, stanice koštane srži, polikromatski eritrociti s mikronukleusom

This content is open access.

Keywords
cisplatin, protransfersom, topička kemoterapija, oštećenje gena, stanice koštane srži, polikromatski eritrociti s mikronukleusom