<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>In the present work, four different spectrophotometric methods for simultaneous estimation of losartan potassium, amlodipine besilate and hydrochlorothiazide in raw materials and in formulations are described. Overlapped data was quantitatively resolved by using chemometric methods, classical least squares (CLS), multiple linear regression (MLR), principal component regression (PCR) and partial least squares (PLS). Calibrations were constructed using the absorption data matrix corresponding to the concentration data matrix, with measurements in the range of 230.5-350.4 nm (Δλ = 0.1 nm) in their zero order spectra. The linearity range was found to be 8-40, 1-5 and 3-15 μg mL<sup>-1</sup> for losartan potassium, amlodipine besilate and hydrochlorothiazide, respectively. The validity of the proposed methods was successfully assessed for analyses of drugs in the various prepared physical mixtures and in tablet formulations.</p></abstract>

In the present work, four different spectrophotometric methods for simultaneous estimation of losartan potassium, amlodipine besilate and hydrochlorothiazide in raw materials and in formulations are described. Overlapped data was quantitatively resolved by using chemometric methods, classical least squares (CLS), multiple linear regression (MLR), principal component regression (PCR) and partial least squares (PLS). Calibrations were constructed using the absorption data matrix corresponding to the concentration data matrix, with measurements in the range of 230.5-350.4 nm (Δλ = 0.1 nm) in their zero order spectra. The linearity range was found to be 8-40, 1-5 and 3-15 μg mL-1 for losartan potassium, amlodipine besilate and hydrochlorothiazide, respectively. The validity of the proposed methods was successfully assessed for analyses of drugs in the various prepared physical mixtures and in tablet formulations.

Simultaneous spectrophotometric determination of losartan potassium, amlodipine besilate and hydrochlorothiazide in pharmaceuticals by chemometric methods

Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur-603319 Kanchipuram District, Tamil Nadu India

Madras Medical College, Chennai-600003, India

C. L. Baid Metha College of Pharmacy, Chennai-600096, Tamil Nadu, India

Acta Pharmaceutica

In the present work, four different spectrophotometric methods for simultaneous estimation of losartan potassium, amlodipine besilate and hydrochlorothiazide...

Simultaneous spectrophotometric determination of losartan potassium, amlodipine besilate and hydrochlorothiazide in pharmaceuticals by chemometric methods

Published Online: Dec 06, 2010

Page range: 141 - 152

DOI: https://doi.org/10.2478/v10007-010-0017-8

Keywords
losartan, amlodipin besilat, hidroklorotiazid, spektrofotometrija, kemometrija

This content is open access.

Simultaneous spectrophotometric determination of losartan potassium, amlodipine besilate and hydrochlorothiazide in pharmaceuticals by chemometric methods

Published Online: Dec 06, 2010

Page range: 141 - 152

DOI: https://doi.org/10.2478/v10007-010-0017-8

Keywordslosartan, amlodipin besilat, hidroklorotiazid, spektrofotometrija, kemometrija

This content is open access.

Keywords
losartan, amlodipin besilat, hidroklorotiazid, spektrofotometrija, kemometrija