<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The analyte and internal standard, nevirapine, were extracted from plasma matrix by liquid-liquid extraction with ethyl acetate. Chromatographic separation was achieved on a C-18 analytical column with an isocratic mobile phase of 15:85 (<italic>V/V</italic>) 10 mmol L-1 ammonium acetate (pH 5.0)/acetonitrile. The atmospheric pressure chemical ionization technique was used for sample ionization in positive ion mode and enhanced selectivity was achieved by tandem mass spectrometric analysis via two multiple reaction monitoring (MRM) transitions, 268.2 → 116.2 for metoprolol and 267.1 → 226.2 for nevirapine, respectively. The assay was validated for human plasma over a concentration range of 1-200 ng mL-1 with the precision and accuracy ranging from 0.9 to 8.8% and 89.9 to 105.8%, respectively.</abstract>

A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The analyte and internal standard, nevirapine, were extracted from plasma matrix by liquid-liquid extraction with ethyl acetate. Chromatographic separation was achieved on a C-18 analytical column with an isocratic mobile phase of 15:85 (V/V) 10 mmol L-1 ammonium acetate (pH 5.0)/acetonitrile. The atmospheric pressure chemical ionization technique was used for sample ionization in positive ion mode and enhanced selectivity was achieved by tandem mass spectrometric analysis via two multiple reaction monitoring (MRM) transitions, 268.2 → 116.2 for metoprolol and 267.1 → 226.2 for nevirapine, respectively. The assay was validated for human plasma over a concentration range of 1-200 ng mL-1 with the precision and accuracy ranging from 0.9 to 8.8% and 89.9 to 105.8%, respectively.

Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry

Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University, Tirupati-517502, India

Bio-Analytical Division, Vimta Labs Limited, Cherlapally Hyderabad-500051, India

Acta Pharmaceutica

A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The...

Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry

Published Online: Dec 06, 2010

Page range: 177 - 184

DOI: https://doi.org/10.2478/v10007-010-0012-0

Keywords
metoprolol, tekućinska kromatografija, spregnuta masena spektrometrija, humana plazma, bioekvivalencija

This content is open access.

Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry

Published Online: Dec 06, 2010

Page range: 177 - 184

DOI: https://doi.org/10.2478/v10007-010-0012-0

Keywordsmetoprolol, tekućinska kromatografija, spregnuta masena spektrometrija, humana plazma, bioekvivalencija

This content is open access.

Keywords
metoprolol, tekućinska kromatografija, spregnuta masena spektrometrija, humana plazma, bioekvivalencija