<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to compare two extraction procedures: dynamic headspace-purge and trap (PT) and headspace solid-phase microextraction (HS-SPME) for gas chromatographic determination of benzene, toluene, ethylbenzene, and isomeric xylenes (BTEX) in urine with photoionization (PID) and mass spectrometric (MS) detection, respectively. Both methods showed linearity in the range of interest [(50-2000) ng L-1], good accuracy (80% to 100 %), and repeatability (RSD≤11 %). Detection limits were in the low ng L-1 level for both methods, although slightly greater sensitivity was found for the PT method. In comparison with PT, HS-SPME was simpler and required less time for analysis.Although the analytical features of both examined methods are appropriate for biomonitoring of environmental exposure to BTEX, only the HS-SPME-GC-MS method is recommended for routine analysis of BTEX in urine. The method was applied for the quantitative analysis of BTEX in urine samples collected from non-smokers (n=10) and smokers (n=10).</abstract>

The aim of this study was to compare two extraction procedures: dynamic headspace-purge and trap (PT) and headspace solid-phase microextraction (HS-SPME) for gas chromatographic determination of benzene, toluene, ethylbenzene, and isomeric xylenes (BTEX) in urine with photoionization (PID) and mass spectrometric (MS) detection, respectively. Both methods showed linearity in the range of interest [(50-2000) ng L-1], good accuracy (80% to 100 %), and repeatability (RSD≤11 %). Detection limits were in the low ng L-1 level for both methods, although slightly greater sensitivity was found for the PT method. In comparison with PT, HS-SPME was simpler and required less time for analysis.Although the analytical features of both examined methods are appropriate for biomonitoring of environmental exposure to BTEX, only the HS-SPME-GC-MS method is recommended for routine analysis of BTEX in urine. The method was applied for the quantitative analysis of BTEX in urine samples collected from non-smokers (n=10) and smokers (n=10).

Comparison Between Dynamic Headspace and Headspace Solid-Phase Microextraction for Gas Chromatography of Btex in Urine

Institute for Medical Research and Occupational Health, Zagreb, Croatia

Archives of Industrial Hygiene and Toxicology

The aim of this study was to compare two extraction procedures: dynamic headspace-purge and trap (PT) and headspace solid-phase microextraction (HS-SPME) for...

Comparison Between Dynamic Headspace and Headspace Solid-Phase Microextraction for Gas Chromatography of Btex in Urine

Published Online: Dec 06, 2007

Page range: 421 - 427

DOI: https://doi.org/10.2478/v10004-007-0035-1

Keywords
GC-MS, PID, ponovljivost, PT, SPME, točnost

This content is open access.

Comparison Between Dynamic Headspace and Headspace Solid-Phase Microextraction for Gas Chromatography of Btex in Urine

Published Online: Dec 06, 2007

Page range: 421 - 427

DOI: https://doi.org/10.2478/v10004-007-0035-1

KeywordsGC-MS, PID, ponovljivost, PT, SPME, točnost

This content is open access.

Keywords
GC-MS, PID, ponovljivost, PT, SPME, točnost