<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The aim of this study was to determine the level of two major mite allergens <italic>Dermatophagoides pteronyssinus</italic> (Der p 1) and <italic>Dermatophagoides farinae</italic> (Der f 1) in 30 urban homes in Zagreb, Croatia, using the enzyme immunoassay with two monoclonal antibodies which has been established as the reference method for indoor allergen analysis. Dust samples were taken by vacuuming a carpeted area and collected on cellulose filters. The ranges of Der p 1 and Der f 1 were (0.1-12.5) μg g-1 (median 0.32 μg g-1) and (0.1-31.2) μg g-1 (median 0.35 μg g-1), respectively. Der p 1 and Der f 1 (>2 μg g-1) associated with increased risk of sensitization to mite allergens were found in approximately 16% homes for each allergen. The sum of allergen (Der p 1 + Der f 1) exceeded the lower threshold in 27% of homes. Analytical evaluation of the ELISA assay showed satisfactory results for precision (intra-assay CV &lt;6.9%, inter-assay CV&lt;13.3%), accuracy (91% to 93%), and sensitivity (2 ng mL-1).The ELISA assay for the measurement of dust mite allergens demonstrated very good analytical characteristics for routine laboratory use, and will provide the essential basis for our future studies of various indoor allergens.</abstract>

The aim of this study was to determine the level of two major mite allergens Dermatophagoides pteronyssinus (Der p 1) and Dermatophagoides farinae (Der f 1) in 30 urban homes in Zagreb, Croatia, using the enzyme immunoassay with two monoclonal antibodies which has been established as the reference method for indoor allergen analysis. Dust samples were taken by vacuuming a carpeted area and collected on cellulose filters. The ranges of Der p 1 and Der f 1 were (0.1-12.5) μg g-1 (median 0.32 μg g-1) and (0.1-31.2) μg g-1 (median 0.35 μg g-1), respectively. Der p 1 and Der f 1 (>2 μg g-1) associated with increased risk of sensitization to mite allergens were found in approximately 16% homes for each allergen. The sum of allergen (Der p 1 + Der f 1) exceeded the lower threshold in 27% of homes. Analytical evaluation of the ELISA assay showed satisfactory results for precision (intra-assay CV <6.9%, inter-assay CV<13.3%), accuracy (91% to 93%), and sensitivity (2 ng mL-1).The ELISA assay for the measurement of dust mite allergens demonstrated very good analytical characteristics for routine laboratory use, and will provide the essential basis for our future studies of various indoor allergens.

Determination of mite Allergens in House Dust Using the Enzyme Immunoassay

Institute for Medical Research and Occupational Health, Zagreb, Croatia

Archives of Industrial Hygiene and Toxicology

{"article-title":"Determination of mite Allergens in House Dust Using the Enzyme Immunoassay"}

The aim of this study was to determine the level of two major mite allergens Dermatophagoides pteronyssinus (Der p 1) and Dermatophagoides farinae (Der f 1) in...

Determination of mite Allergens in House Dust Using the Enzyme Immunoassay

Published Online: Dec 06, 2007

Page range: 413 - 419

DOI: https://doi.org/10.2478/v10004-007-0034-2

Keywords
alergeni unutarnjih prostora, Der f 1, Der p 1, ELISA, grinje kućne prašine

This content is open access.

Determination of mite Allergens in House Dust Using the Enzyme Immunoassay

Published Online: Dec 06, 2007

Page range: 413 - 419

DOI: https://doi.org/10.2478/v10004-007-0034-2

Keywordsalergeni unutarnjih prostora, Der f 1, Der p 1, ELISA, grinje kućne prašine

This content is open access.

Keywords
alergeni unutarnjih prostora, Der f 1, Der p 1, ELISA, grinje kućne prašine