<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We introduce two level-set method approaches to segmentation of 2D macrophage images. The first one is based on the Otsu thresholding and the second one on the information entropy thresholding, both followed by the classical subjective surface (SUBSURF) method. Results of both methods are compared with the semi-automatic Lagrangian method in which the segmentation curve evolves along the edge of the macrophage and it is controlled by an expert user. We present the comparison of all three methods with respect to the Hausdorff distance of resulting segmentation curves and we compare also their perimeter and enclosed area. We show that accuracy of the automatic SUBSURF method is comparable to the results of the semi-automatic Lagrangian segmentation.</p></abstract>

We introduce two level-set method approaches to segmentation of 2D macrophage images. The first one is based on the Otsu thresholding and the second one on the information entropy thresholding, both followed by the classical subjective surface (SUBSURF) method. Results of both methods are compared with the semi-automatic Lagrangian method in which the segmentation curve evolves along the edge of the macrophage and it is controlled by an expert user. We present the comparison of all three methods with respect to the Hausdorff distance of resulting segmentation curves and we compare also their perimeter and enclosed area. We show that accuracy of the automatic SUBSURF method is comparable to the results of the semi-automatic Lagrangian segmentation.

Macrophage Image Segmentation by Thresholding and Subjective Surface Method

Department of Mathematics and Descriptive Geometry

Slovak University of Technology in Bratislava

Tatra Mountains Mathematical Publications

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Macrophage Image Segmentation by Thresholding and Subjective Surface Method"}

We introduce two level-set method approaches to segmentation of 2D macrophage images. The first one is based on the Otsu thresholding and the second one on the...

Macrophage Image Segmentation by Thresholding and Subjective Surface Method

Published Online: Apr 24, 2020

Page range: 103 - 120

Received: Jul 04, 2019

DOI: https://doi.org/10.2478/tmmp-2020-0007

Keywords
image processing, segmentation, level-set methods, finite volume methods, thresholding, macrophages

© 2020 Seol Ah Park et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Macrophage Image Segmentation by Thresholding and Subjective Surface Method

Published Online: Apr 24, 2020

Page range: 103 - 120

Received: Jul 04, 2019

DOI: https://doi.org/10.2478/tmmp-2020-0007

Keywordsimage processing, segmentation, level-set methods, finite volume methods, thresholding, macrophages

© 2020 Seol Ah Park et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
image processing, segmentation, level-set methods, finite volume methods, thresholding, macrophages