<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of this study is to investigate the chemical composition of methanol extracts of the lichens Pleurosticta acetabulum and Cladonia subulata and their antioxidant, and anticancer activities. The phytochemical analysis of the extracts of lichens was determined by HPLC-UV method. The predominant phenolic compounds in these extracts were norstictic acid and salazinic acids in P. acetabulum, while hypoprotocetraric acid and fumarprotocetraric acid were the major metabolites detected in C. subulata. Total phenolics and flavonoids in the extracts were determined spectrophotometrically, with the varied amount from 21.31 to 73.45 mg GA/g and from 8.48 to 15.42 mg RU/g, respectively. The lichen extracts showed comparable and strong antioxidant activity, exhibited higher DPPH and hydroxyl radical scavengings, inhibitory activity towards lipid peroxidation and reducing power. Cytotoxic effects of lichens were tested against HeLa S3 and LS174 cell lines using MTT method.The cytotoxic effects of P. acetabulum and C. subulata extracts toward two cancer cell lines were in the range from 39.17 to >200 μg/mL IC50 value. The present study showed that the tested extracts of lichens demonstrated important antioxidant and anticancer effects. That suggests that these lichens can be used as new sources of the natural antioxidants and anti-cancer compounds.</p>
</abstract>

The aim of this study is to investigate the chemical composition of methanol extracts of the lichens Pleurosticta acetabulum and Cladonia subulata and their antioxidant, and anticancer activities. The phytochemical analysis of the extracts of lichens was determined by HPLC-UV method. The predominant phenolic compounds in these extracts were norstictic acid and salazinic acids in P. acetabulum, while hypoprotocetraric acid and fumarprotocetraric acid were the major metabolites detected in C. subulata. Total phenolics and flavonoids in the extracts were determined spectrophotometrically, with the varied amount from 21.31 to 73.45 mg GA/g and from 8.48 to 15.42 mg RU/g, respectively. The lichen extracts showed comparable and strong antioxidant activity, exhibited higher DPPH and hydroxyl radical scavengings, inhibitory activity towards lipid peroxidation and reducing power. Cytotoxic effects of lichens were tested against HeLa S3 and LS174 cell lines using MTT method.The cytotoxic effects of P. acetabulum and C. subulata extracts toward two cancer cell lines were in the range from 39.17 to >200 μg/mL IC50 value. The present study showed that the tested extracts of lichens demonstrated important antioxidant and anticancer effects. That suggests that these lichens can be used as new sources of the natural antioxidants and anti-cancer compounds.

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens <italic>Pleurosticta Acetabulum </italic>and <italic>Cladonia Subulata</italic>

University of Kragujevac, Faculty of Medical Sciences

University of Kragujevac, Faculty of Science

Faculty of Medicine, Department of Biology and Human Genetics Nis

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens and 

The aim of this study is to investigate the chemical composition of methanol extracts of the lichens Pleurosticta acetabulum and Cladonia subulata and their...

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta Acetabulum and Cladonia Subulata

Published Online: Jan 02, 2024

Page range: 305 - 314

Received: May 10, 2020

Accepted: Jul 15, 2020

DOI: https://doi.org/10.2478/sjecr-2020-0057

Keywords
Antioxidant activity, chemical composition, cytotoxic activity, lichen, phenols

© 2020 Jovica Tomovic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta Acetabulum and Cladonia Subulata

Published Online: Jan 02, 2024

Page range: 305 - 314

Received: May 10, 2020

Accepted: Jul 15, 2020

DOI: https://doi.org/10.2478/sjecr-2020-0057

KeywordsAntioxidant activity, chemical composition, cytotoxic activity, lichen, phenols

© 2020 Jovica Tomovic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Antioxidant activity, chemical composition, cytotoxic activity, lichen, phenols