<abstract xmlns="http://www.w3.org/1999/xhtml">Apoptosis inhibition in chronic lymphocytic leukemia (CLL) is one of the most important mechanism in the disease onset, progression and therapy response and is dependent of interaction with different microenvironments.Aim of our paper is to determine expression of antiapoptoic proteins mcl-1 and bcl-2 in CLL cells isolated from two different compartments (peripheral blood and bone marrow) and its relation to percent of apoptotic cells and concentration of growth factors (FGF and VEGF).Our results showed that peripheral blood CLL lymphocytes have lower apoptotic rate then those isolated from bone marrow, though bone marrow CLL lymphocytes express higher levels of antipoptotic proteins bcl-2 and mcl-1. In bone marrow FGF concentration is 10-fold higher then in patients plasma but has an limited impact on mcl-1 expression. In contrary, VEGF concentration is higher in peripheral blood and corelate with percent of apoptotic cells and mcl-1 expression in this compartment.CLL cells derived from two different microenvironmets acts differently when tested for apoptosis „ex vivo“. In peripheral blood apoptosis is strongly connected with expression of antiapoptoic proteins (mcl-1 and bcl-2) and growth factors, but not in bone marrow.</abstract>

Apoptosis inhibition in chronic lymphocytic leukemia (CLL) is one of the most important mechanism in the disease onset, progression and therapy response and is dependent of interaction with different microenvironments.Aim of our paper is to determine expression of antiapoptoic proteins mcl-1 and bcl-2 in CLL cells isolated from two different compartments (peripheral blood and bone marrow) and its relation to percent of apoptotic cells and concentration of growth factors (FGF and VEGF).Our results showed that peripheral blood CLL lymphocytes have lower apoptotic rate then those isolated from bone marrow, though bone marrow CLL lymphocytes express higher levels of antipoptotic proteins bcl-2 and mcl-1. In bone marrow FGF concentration is 10-fold higher then in patients plasma but has an limited impact on mcl-1 expression. In contrary, VEGF concentration is higher in peripheral blood and corelate with percent of apoptotic cells and mcl-1 expression in this compartment.CLL cells derived from two different microenvironmets acts differently when tested for apoptosis „ex vivo“. In peripheral blood apoptosis is strongly connected with expression of antiapoptoic proteins (mcl-1 and bcl-2) and growth factors, but not in bone marrow.

Antiapoptotic Proteins mcl-1 and bcl-2 as well as Growth Factors FGF and VEGF Influence Survival of Peripheral Blood and Bone Marrow Chronic Lymphocytic Leukemia Cells

Faculty of Medical Sciences, Department of Internal medicine

Faculty of Medical Sciences, Department of Microbiology and immunology

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Apoptosis inhibition in chronic lymphocytic leukemia (CLL) is one of the most important mechanism in the disease onset, progression and therapy response and is...

Antiapoptotic Proteins mcl-1 and bcl-2 as well as Growth Factors FGF and VEGF Influence Survival of Peripheral Blood and Bone Marrow Chronic Lymphocytic Leukemia Cells

Published Online: Aug 27, 2020

Page range: 133 - 139

Received: Jun 12, 2018

Accepted: Jul 18, 2018

DOI: https://doi.org/10.2478/sjecr-2018-0025

Keywords
CLL, apoptosis, mcl-1, VEGF, FGF

© 2020 Predrag Djurdjevic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antiapoptotic Proteins mcl-1 and bcl-2 as well as Growth Factors FGF and VEGF Influence Survival of Peripheral Blood and Bone Marrow Chronic Lymphocytic Leukemia Cells

Published Online: Aug 27, 2020

Page range: 133 - 139

Received: Jun 12, 2018

Accepted: Jul 18, 2018

DOI: https://doi.org/10.2478/sjecr-2018-0025

KeywordsCLL, apoptosis, mcl-1, VEGF, FGF

© 2020 Predrag Djurdjevic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
CLL, apoptosis, mcl-1, VEGF, FGF