<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Introduction</bold>: Skin flap application in the clinical practice is restricted due to the ischemic damage and flap necrosis. Rosmarinus oil has been shown to improve a skin flap survival. In the present work we studied the role of mammalian target of rapamycin (mTOR) signaling on rosmarinus-induced flap protection. <bold>Methods:</bold> A flap surgery was performed on Sprauge-Dawley rats (8 cm in by 3 cm). A week before and a week after the surgery the flaps were treated with topical rosmarinus oil (twice per day). Rapamycin (m-TOR inhibitor) was administered 30 minutes before the flap surgery in rosmarinus-treated or not treated groups. A week after the surgery the malondialdehyde (MDA) contents, myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, expression of Bax, Bcl-2, mTOR and p-mTOR were measured in the flap tissue. <bold>Results:</bold> Topical application of the rosmarinus increased the flap survival (p&lt;0.05), anti-oxidative enzyme activity (SOD, p&lt;0.05) and anti-apoptotic protein Bcl-2 expression. Rosmarinus treatment decreased the flap MDA content, MPO activity, and pro-apoptotic protein Bax expression (p&lt;0.05). Rosmarinus topical application did not change mTOR expression and phosphorylation in the flap tissue. Expression of p-mTOR in rosmarinus treated group was suppressed by rapamycin pre-treatment, which also abolished rosmarinus effects on the flap survival (p&lt;0.05). <bold>Conclusion:</bold> These data suggested p-mTOR dependent mechanism in rosmarinus-induced flap survival.</p></abstract>

Introduction: Skin flap application in the clinical practice is restricted due to the ischemic damage and flap necrosis. Rosmarinus oil has been shown to improve a skin flap survival. In the present work we studied the role of mammalian target of rapamycin (mTOR) signaling on rosmarinus-induced flap protection. Methods: A flap surgery was performed on Sprauge-Dawley rats (8 cm in by 3 cm). A week before and a week after the surgery the flaps were treated with topical rosmarinus oil (twice per day). Rapamycin (m-TOR inhibitor) was administered 30 minutes before the flap surgery in rosmarinus-treated or not treated groups. A week after the surgery the malondialdehyde (MDA) contents, myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, expression of Bax, Bcl-2, mTOR and p-mTOR were measured in the flap tissue. Results: Topical application of the rosmarinus increased the flap survival (p<0.05), anti-oxidative enzyme activity (SOD, p<0.05) and anti-apoptotic protein Bcl-2 expression. Rosmarinus treatment decreased the flap MDA content, MPO activity, and pro-apoptotic protein Bax expression (p<0.05). Rosmarinus topical application did not change mTOR expression and phosphorylation in the flap tissue. Expression of p-mTOR in rosmarinus treated group was suppressed by rapamycin pre-treatment, which also abolished rosmarinus effects on the flap survival (p<0.05). Conclusion: These data suggested p-mTOR dependent mechanism in rosmarinus-induced flap survival.

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Department of nutrition, faculty of medicine

Physiology Research Center, Faculty of Medicine

Department of Clinical Nutrition, Faculty of Nutrition & Food Technology

Shahid Beheshti University of Medical Sciences

Serbian Journal of Dermatology and Venereology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway"}

Introduction: Skin flap application in the clinical practice is restricted due to the ischemic damage and flap necrosis. Rosmarinus oil has been shown to...

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Published Online: Mar 28, 2019

Page range: 113 - 118

Received: Sep 03, 2018

Accepted: Dec 08, 2019

DOI: https://doi.org/10.2478/sjdv-2018-0016

Keywords
Rosmarinus, Plant Oils, Ischemia, Reperfusion Injury, Surgical Flaps, Rats, Sprague Dawley, Graft Survival, Necrosis, Apoptosis

© 2018 Zeinab Javid et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Published Online: Mar 28, 2019

Page range: 113 - 118

Received: Sep 03, 2018

Accepted: Dec 08, 2019

DOI: https://doi.org/10.2478/sjdv-2018-0016

KeywordsRosmarinus, Plant Oils, Ischemia, Reperfusion Injury, Surgical Flaps, Rats, Sprague Dawley, Graft Survival, Necrosis, Apoptosis

© 2018 Zeinab Javid et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Rosmarinus, Plant Oils, Ischemia, Reperfusion Injury, Surgical Flaps, Rats, Sprague Dawley, Graft Survival, Necrosis, Apoptosis