<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Chionanthus retusus</italic> is one of the well-known ornamental trees in East Asia and America. Not only its value in the market but also had the potential as a source for producing antioxidant. However, due to uncontrolled exploitation, the number of wild <italic>C. retusus</italic> in China is decreasing rapidly. The genetic study of <italic>C. retusus</italic> is limited. In order to investigate the genetic diversity and the distribution of <italic>C. retusus</italic> in China, 47 samples from 8 different provinces have been sequenced via restriction site-associated DNA sequencing (RAD-seq). Totally, 31, 402 high-quality single nucleotide polymorphisms (SNP) were obtained. According to the phylogenetic tree and the principal component analysis, the samples were divided into four populations, including 3 major populations and 1 hybrid population. Population1 were the samples from Jiangsu and Yunnan province and the Population2 were mainly from northern and northeast of China including Liaoning and Hubei province, while the Population4 were from Shandong and Henan province, which were in central China. As the admixture showed, the population3 were the offspring of the other 3 populations by hybridization. The mean heterozygosity of Chinese Fringe tree from different province is 0.42 %, with the highest heterozygosity, which is as high as 0.63 %, from Jiangsu province and the lowest heterozygosity, which is only 0.19, from Henan province. This is the first report about the genetic diversity and relationship of <italic>Chionanthus retusus</italic>, which will provide value information for further genetic study, genomic study, conservation and breeding.</p>
</abstract>

Chionanthus retusus is one of the well-known ornamental trees in East Asia and America. Not only its value in the market but also had the potential as a source for producing antioxidant. However, due to uncontrolled exploitation, the number of wild C. retusus in China is decreasing rapidly. The genetic study of C. retusus is limited. In order to investigate the genetic diversity and the distribution of C. retusus in China, 47 samples from 8 different provinces have been sequenced via restriction site-associated DNA sequencing (RAD-seq). Totally, 31, 402 high-quality single nucleotide polymorphisms (SNP) were obtained. According to the phylogenetic tree and the principal component analysis, the samples were divided into four populations, including 3 major populations and 1 hybrid population. Population1 were the samples from Jiangsu and Yunnan province and the Population2 were mainly from northern and northeast of China including Liaoning and Hubei province, while the Population4 were from Shandong and Henan province, which were in central China. As the admixture showed, the population3 were the offspring of the other 3 populations by hybridization. The mean heterozygosity of Chinese Fringe tree from different province is 0.42 %, with the highest heterozygosity, which is as high as 0.63 %, from Jiangsu province and the lowest heterozygosity, which is only 0.19, from Henan province. This is the first report about the genetic diversity and relationship of Chionanthus retusus, which will provide value information for further genetic study, genomic study, conservation and breeding.

SNPs Detection and Genetic Analysis of <italic>Chionanthus retusus</italic> via Genotyping-by-Sequencing

Xinyang Agricultural and Forestry University

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

SNPs Detection and Genetic Analysis of  via Genotyping-by-Sequencing

Chionanthus retusus is one of the well-known ornamental trees in East Asia and America. Not only its value in the market but also had the potential as a source...

SNPs Detection and Genetic Analysis of Chionanthus retusus via Genotyping-by-Sequencing

Published Online: Jul 16, 2023

Page range: 118 - 125

DOI: https://doi.org/10.2478/sg-2023-0012

Keywords
genotyping-by-sequencing, restriction site-associated DNA sequencing, single nucleotide polymorphism, genetic diversity

© 2023 Song Huang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

SNPs Detection and Genetic Analysis of Chionanthus retusus via Genotyping-by-Sequencing

Published Online: Jul 16, 2023

Page range: 118 - 125

DOI: https://doi.org/10.2478/sg-2023-0012

Keywordsgenotyping-by-sequencing, restriction site-associated DNA sequencing, single nucleotide polymorphism, genetic diversity

© 2023 Song Huang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
genotyping-by-sequencing, restriction site-associated DNA sequencing, single nucleotide polymorphism, genetic diversity