<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Juglans</italic> is a genus of ca. 20 species divided into four sections (<italic>Rhysocaryon, Cardiocaryon, Trachycaryon</italic> and <italic>Juglans</italic>) with a broad geographic distribution which is known for producing edible nuts and high-quality wood. Hybrid <italic>Juglans</italic> trees are becoming increasingly popular for forestry purposes in Europe because of their fast growth and adaptability. In this work, DNA was isolated from leaves of eighty-five individuals of <italic>Juglans</italic> species and hybrids and evaluated at eight nSSR and seven EST-SSR markers. The markers were able to differentiate all trees on an individual basis, and further, gave indications of polyploidy in some samples. Consequently, fifty-nine samples were then checked for ploidy by flow cytometry. Genetic structure of the population was evaluated with the most probable number of groups being K=3, which agrees with the four sections of the genus <italic>Juglans</italic>, where <italic>Cardiocaryon</italic> and <italic>Trachycaryon</italic> are genetically very similar and thus comprise one group. These results agree with other research using SSRs in <italic>Juglans</italic>. Flow cytometry revealed thirteen triploid individuals, where twelve are in agreement with the marker study, one was only detected by flow cytometry.</p>
</abstract>

Juglans is a genus of ca. 20 species divided into four sections (Rhysocaryon, Cardiocaryon, Trachycaryon and Juglans) with a broad geographic distribution which is known for producing edible nuts and high-quality wood. Hybrid Juglans trees are becoming increasingly popular for forestry purposes in Europe because of their fast growth and adaptability. In this work, DNA was isolated from leaves of eighty-five individuals of Juglans species and hybrids and evaluated at eight nSSR and seven EST-SSR markers. The markers were able to differentiate all trees on an individual basis, and further, gave indications of polyploidy in some samples. Consequently, fifty-nine samples were then checked for ploidy by flow cytometry. Genetic structure of the population was evaluated with the most probable number of groups being K=3, which agrees with the four sections of the genus Juglans, where Cardiocaryon and Trachycaryon are genetically very similar and thus comprise one group. These results agree with other research using SSRs in Juglans. Flow cytometry revealed thirteen triploid individuals, where twelve are in agreement with the marker study, one was only detected by flow cytometry.

Clonal Differentiation and Identification of Polyploids in <italic>Juglans</italic> hybrids

Department of Forest Genetics and Forest Tree Breeding

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Clonal Differentiation and Identification of Polyploids in  hybrids

Juglans is a genus of ca. 20 species divided into four sections (Rhysocaryon, Cardiocaryon, Trachycaryon and Juglans) with a broad geographic distribution...

Clonal Differentiation and Identification of Polyploids in Juglans hybrids

Published Online: Jun 16, 2023

Page range: 72 - 80

DOI: https://doi.org/10.2478/sg-2023-0007

Keywords
Walnut, PCR, Hybrid, × intermedia, triploid, marker, breeding

© 2023 Winston Beck et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Clonal Differentiation and Identification of Polyploids in Juglans hybrids

Published Online: Jun 16, 2023

Page range: 72 - 80

DOI: https://doi.org/10.2478/sg-2023-0007

KeywordsWalnut, PCR, Hybrid, × intermedia, triploid, marker, breeding

© 2023 Winston Beck et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Walnut, PCR, Hybrid, × intermedia, triploid, marker, breeding