<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Ailanthus altissima</italic> is a deciduous tree native to China and introduced to other parts of the world as an ornamental plant. It exhibits resistance to both abiotic and biotic stress factors and has various pharmacological effects and strong allelopathy, generating significant research interests. However, the genome sequence of this species has not been reported, limiting its research development. The purpose of the study was to determine the genome size and characteristics of <italic>A. altissima</italic> to conduct its genomic survey. Next-generation sequencing and K-mer analysis were employed to measure the genome size of <italic>A. altissima</italic>. Overall, a total of 61.93 Gb high-quality clean data were acquired, representing approximately 64.09× coverage of the <italic>A. altissima</italic> genome. The genomic characteristics of <italic>A. altissima</italic> include a genome size of 966.38 Mbp, a heterozygosis rate of 0.78 %, and a repeat rate of 41.22 %. A total of 735,179 genomic SSRs markers were identified based on genome survey sequences. Alignment analysis showed that <italic>A. altissima</italic> was closely related to <italic>Citrus sinensis</italic> and <italic>Leitneria florida-na</italic>. This study provides basic information for future whole-genomic sequencing of <italic>A. altissima</italic>. This will facilitate a knowledge of the population structure, genetic diversity, long distance-gene transfer, and pollen-based gene flow analyses of <italic>A. altissima</italic> populations from its known distribution ranges in China, focusing on planted and natural forest stands.</p>
</abstract>

Ailanthus altissima is a deciduous tree native to China and introduced to other parts of the world as an ornamental plant. It exhibits resistance to both abiotic and biotic stress factors and has various pharmacological effects and strong allelopathy, generating significant research interests. However, the genome sequence of this species has not been reported, limiting its research development. The purpose of the study was to determine the genome size and characteristics of A. altissima to conduct its genomic survey. Next-generation sequencing and K-mer analysis were employed to measure the genome size of A. altissima. Overall, a total of 61.93 Gb high-quality clean data were acquired, representing approximately 64.09× coverage of the A. altissima genome. The genomic characteristics of A. altissima include a genome size of 966.38 Mbp, a heterozygosis rate of 0.78 %, and a repeat rate of 41.22 %. A total of 735,179 genomic SSRs markers were identified based on genome survey sequences. Alignment analysis showed that A. altissima was closely related to Citrus sinensis and Leitneria florida-na. This study provides basic information for future whole-genomic sequencing of A. altissima. This will facilitate a knowledge of the population structure, genetic diversity, long distance-gene transfer, and pollen-based gene flow analyses of A. altissima populations from its known distribution ranges in China, focusing on planted and natural forest stands.

Genome survey sequencing of <italic>Ailanthus altissima</italic> and identification of simple sequence repeat (SSR) markers

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genome survey sequencing of  and identification of simple sequence repeat (SSR) markers

Ailanthus altissima is a deciduous tree native to China and introduced to other parts of the world as an ornamental plant. It exhibits resistance to both...

Genome survey sequencing of Ailanthus altissima and identification of simple sequence repeat (SSR) markers

Published Online: Aug 08, 2022

Page range: 47 - 53

DOI: https://doi.org/10.2478/sg-2022-0006

Keywords
Ailanthus altissima, genome survey, next-generation sequencing, genomic SSR markers

© 2022 Yaping Ma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genome survey sequencing of Ailanthus altissima and identification of simple sequence repeat (SSR) markers

Published Online: Aug 08, 2022

Page range: 47 - 53

DOI: https://doi.org/10.2478/sg-2022-0006

KeywordsAilanthus altissima, genome survey, next-generation sequencing, genomic SSR markers

© 2022 Yaping Ma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Ailanthus altissima, genome survey, next-generation sequencing, genomic SSR markers