<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Glyptostrobus pensilis</italic> (Cupressaceae) is a critically endangered conifer which occurs in China, Laos, and Vietnam where it is only known from a few populations. Here we aim to develop microsatellite markers which can be used to study the genetic variation within this species. Using transcriptome data we tested 170 SSR loci for polymorphism in 16 samples. Twenty-three loci were polymorphic and selected for the genetic analysis of 83 individuals from three Chinese populations. The number of alleles per locus and population ranged from one to eight, the observed and expected heterozygosity from <italic>H</italic><italic>o</italic> = 0.00-1.00 and <italic>H</italic><italic>e</italic> = 0.00-0.83, respectively. Fifteen loci deviated from Hardy-Weinberg equilibrium in at least one of the three populations. The majority of loci could also be successfully amplified in four related species, namely <italic>Cryptomeria fortunei, Taxodium distichum, Taxodium ascendens</italic> and <italic>Cunninghamia lanceolata</italic>. These developed microsatellites are suitable for population genetic studies of <italic>Glyptostrobus pensilis</italic> and related species.</abstract>

Glyptostrobus pensilis (Cupressaceae) is a critically endangered conifer which occurs in China, Laos, and Vietnam where it is only known from a few populations. Here we aim to develop microsatellite markers which can be used to study the genetic variation within this species. Using transcriptome data we tested 170 SSR loci for polymorphism in 16 samples. Twenty-three loci were polymorphic and selected for the genetic analysis of 83 individuals from three Chinese populations. The number of alleles per locus and population ranged from one to eight, the observed and expected heterozygosity from Ho = 0.00-1.00 and He = 0.00-0.83, respectively. Fifteen loci deviated from Hardy-Weinberg equilibrium in at least one of the three populations. The majority of loci could also be successfully amplified in four related species, namely Cryptomeria fortunei, Taxodium distichum, Taxodium ascendens and Cunninghamia lanceolata. These developed microsatellites are suitable for population genetic studies of Glyptostrobus pensilis and related species.

Development of microsatellite markers for the critically endangered conifer <italic>Glyptostrobus pensilis</italic> (Cupressaceae) using transcriptome data

Central South University of Forestry and Technology

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of microsatellite markers for the critically endangered conifer  (Cupressaceae) using transcriptome data

Glyptostrobus pensilis (Cupressaceae) is a critically endangered conifer which occurs in China, Laos, and Vietnam where it is only known from a few...

Development of microsatellite markers for the critically endangered conifer Glyptostrobus pensilis (Cupressaceae) using transcriptome data

Published Online: Apr 09, 2019

Page range: 41 - 44

DOI: https://doi.org/10.2478/sg-2019-0007

Keywords
: Glyptostrobus pensilis, next-generation sequencing, Expressed Sequence Tag (EST), simple sequence repeat (SSR) markers

© 2019 Xin-Yu Li et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Supplementary Material Details

Supplementary Material Details

Supplementary Material Details

Development of microsatellite markers for the critically endangered conifer Glyptostrobus pensilis (Cupressaceae) using transcriptome data

Published Online: Apr 09, 2019

Page range: 41 - 44

DOI: https://doi.org/10.2478/sg-2019-0007

Keywords: Glyptostrobus pensilis, next-generation sequencing, Expressed Sequence Tag (EST), simple sequence repeat (SSR) markers

© 2019 Xin-Yu Li et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Supplementary Material Details

Supplementary Material Details

Supplementary Material Details

Keywords
: Glyptostrobus pensilis, next-generation sequencing, Expressed Sequence Tag (EST), simple sequence repeat (SSR) markers