<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>This study was aimed to explore the nanoparticle synthesizing properties of a silver resistant Bacillus sp. isolated from a marine water sample. The 16SrDNA sequence analysis of the isolate proved it as a Bacillus strain. Very interestingly, the isolate was found to have the ability to form intracellular silver nanoparticles at room temperature within 24 hours. This was confirmed by the UV-Vis absorption analysis which showed a peak at 430 nm corresponding to the plasmon absorbance of silver nanoparticles. Further characterization of the nanoparticles was carried out by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analysis. The presence of silver nanoparticles with the size less than 100 nm was confirmed. These particles were found to be extremely stable as confirmed by the TEM analysis after three months of purification. So, the current study is the demonstration of an efficient synthesis of stable silver nanoparticles by a marine Bacillus strain.</p></abstract>

This study was aimed to explore the nanoparticle synthesizing properties of a silver resistant Bacillus sp. isolated from a marine water sample. The 16SrDNA sequence analysis of the isolate proved it as a Bacillus strain. Very interestingly, the isolate was found to have the ability to form intracellular silver nanoparticles at room temperature within 24 hours. This was confirmed by the UV-Vis absorption analysis which showed a peak at 430 nm corresponding to the plasmon absorbance of silver nanoparticles. Further characterization of the nanoparticles was carried out by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analysis. The presence of silver nanoparticles with the size less than 100 nm was confirmed. These particles were found to be extremely stable as confirmed by the TEM analysis after three months of purification. So, the current study is the demonstration of an efficient synthesis of stable silver nanoparticles by a marine Bacillus strain.

Biosynthesis of silver nanoparticles by a Bacillus sp. of marine origin

School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, India, 686 560

Plant Molecular Biology, Rajiv Gandhi Centre for Biotechnology, Thycaud (PO), Poojappura, Thiruvananthapuram, Kerala, India, 695 014

Materials Science-Poland

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Biosynthesis of silver nanoparticles by a Bacillus sp. of marine origin"}

This study was aimed to explore the nanoparticle synthesizing properties of a silver resistant Bacillus sp. isolated from a marine water sample. The 16SrDNA...

Biosynthesis of silver nanoparticles by a Bacillus sp. of marine origin

Published Online: Apr 20, 2013

Page range: 173 - 179

DOI: https://doi.org/10.2478/s13536-012-0085-1

Keywords
silver nanoparticle, biosynthesis, purification, marine Bacillus sp.

© 2013 Wroclaw University of Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Biosynthesis of silver nanoparticles by a Bacillus sp. of marine origin

Published Online: Apr 20, 2013

Page range: 173 - 179

DOI: https://doi.org/10.2478/s13536-012-0085-1

Keywordssilver nanoparticle, biosynthesis, purification, marine Bacillus sp.

© 2013 Wroclaw University of Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
silver nanoparticle, biosynthesis, purification, marine Bacillus sp.