<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The infectivity of Toxocara cati larvae in mouse tissues, following storage at 4 °C and −25 °C, was measured using a bioassay in mice. Tissues of donor mice infected with 30-day-old T. cati larvae were stored at 4 °C for 7 and 14 days, and at −25 °C for 24 hours, then tissue larvae were inoculated into recipient mice (n = 6/group). After 15 days, larval burden in recipient mice was assessed by digestion. In the control group, a mean of 42.0 % of the inoculated larvae were established in mice. Storage of tissues at 4 °C for 7 and 14 days did not affect larval infectivity; means of 43.5 % and 37.4 % of inoculated larvae, respectively, were established in mice. Larvae exposed to −25 °C for 24 hours did not establish in mice, except for one larva recovered from a single mouse. These results may support the potential of tissue larvae in livestock animals as a causative agent of human toxocarosis.</p></abstract>

The infectivity of Toxocara cati larvae in mouse tissues, following storage at 4 °C and −25 °C, was measured using a bioassay in mice. Tissues of donor mice infected with 30-day-old T. cati larvae were stored at 4 °C for 7 and 14 days, and at −25 °C for 24 hours, then tissue larvae were inoculated into recipient mice (n = 6/group). After 15 days, larval burden in recipient mice was assessed by digestion. In the control group, a mean of 42.0 % of the inoculated larvae were established in mice. Storage of tissues at 4 °C for 7 and 14 days did not affect larval infectivity; means of 43.5 % and 37.4 % of inoculated larvae, respectively, were established in mice. Larvae exposed to −25 °C for 24 hours did not establish in mice, except for one larva recovered from a single mouse. These results may support the potential of tissue larvae in livestock animals as a causative agent of human toxocarosis.

Effect of low temperatures on the infectivity of Toxocara cati larvae parasitized in mouse tissues

Laboratory of Parasitology, Department of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara Chuo-ku, Kanagawa, 252-5201, Japan

Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The infectivity of Toxocara cati larvae in mouse tissues, following storage at 4 °C and −25 °C, was measured using a bioassay in mice. Tissues of donor mice...

Effect of low temperatures on the infectivity of Toxocara cati larvae parasitized in mouse tissues

Published Online: Aug 31, 2013

Page range: 83 - 86

DOI: https://doi.org/10.2478/s11687-013-0112-8

Keywords
Toxocara cati, tissue larvae, cold tolerance, zoonosis

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of low temperatures on the infectivity of Toxocara cati larvae parasitized in mouse tissues

Published Online: Aug 31, 2013

Page range: 83 - 86

DOI: https://doi.org/10.2478/s11687-013-0112-8

KeywordsToxocara cati, tissue larvae, cold tolerance, zoonosis

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Toxocara cati, tissue larvae, cold tolerance, zoonosis