<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Toxocariasis is the clinical syndrome caused by infection of zoonotic roundworms of dogs (Toxocara canis) or cats (Toxocara cati). Current research on the immunology and pathology aspects of toxocariasis requires Toxocara second stage larvae and a ready source of excretory-secretory (ES) antigens. We cultured eleven pairs of both sexes of Toxocara cati adult worms maintained in RPMI 1640 medium in order to evaluate the amounts and duration of egg laying. At the first day and last day (day 19), the mean egg counts were 9300 and 250 eggs/ml, respectively. These results showed that this culture method is very appropriate for collection of pure oviposited eggs and/or production of adult ES antigens of Toxocara cati that could be used for immunological diagnosis of toxocariasis.</p></abstract>

Toxocariasis is the clinical syndrome caused by infection of zoonotic roundworms of dogs (Toxocara canis) or cats (Toxocara cati). Current research on the immunology and pathology aspects of toxocariasis requires Toxocara second stage larvae and a ready source of excretory-secretory (ES) antigens. We cultured eleven pairs of both sexes of Toxocara cati adult worms maintained in RPMI 1640 medium in order to evaluate the amounts and duration of egg laying. At the first day and last day (day 19), the mean egg counts were 9300 and 250 eggs/ml, respectively. These results showed that this culture method is very appropriate for collection of pure oviposited eggs and/or production of adult ES antigens of Toxocara cati that could be used for immunological diagnosis of toxocariasis.

In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Department of Parasitology, Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, Kobe, Japan

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Toxocariasis is the clinical syndrome caused by infection of zoonotic roundworms of dogs (Toxocara canis) or cats (Toxocara cati). Current research on the...

In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

Published Online: Apr 25, 2009

Page range: 28 - 30

DOI: https://doi.org/10.2478/s11687-009-0005-z

Keywords
Toxocara cati, cultivation, in vitro, Adult worms

© 2009 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

Published Online: Apr 25, 2009

Page range: 28 - 30

DOI: https://doi.org/10.2478/s11687-009-0005-z

KeywordsToxocara cati, cultivation, in vitro, Adult worms

© 2009 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Toxocara cati, cultivation, in vitro, Adult worms