<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Four entomopathogenic nematode species (Steinernema feltiae, S. carpocapsae, Heterorhabditis bacteriophora, and H. megidis) were tested in a laboratory bioassay for the efficacy of these pathogens in controlling the larvae and adults of the Colorado potato beetle, Leptinotarsa decemlineata. The main aim of the study was to develop an efficient sustainable control method against the pest. With this we could develop a strategy of potato production with the intention of diminishing or even preventing the appearance of pest resistance to insecticides. The activity of these biological agents was assessed at three different temperatures (15, 20, and 25 °C) and three concentrations (200, 1000, and 2000 infective juveniles per individual). Mortality of three stages (young and old larvae and adults) was determined 2, 4, and 7 days after treatment. At 15 °C entomopathogenic nematodes showed the lowest efficacy against all insect stages. No significant differences in efficacy was determined at 20 and 25 °C as all nematodes caused prompt death of all stages. At all temperatures young larvae were most susceptible. However, when controlling overwintered adults for the purpose of preventing the mass appearance of Colorado potato beetle, we recommend an application of higher concentrations of S. feltiae suspension.</p></abstract>

Four entomopathogenic nematode species (Steinernema feltiae, S. carpocapsae, Heterorhabditis bacteriophora, and H. megidis) were tested in a laboratory bioassay for the efficacy of these pathogens in controlling the larvae and adults of the Colorado potato beetle, Leptinotarsa decemlineata. The main aim of the study was to develop an efficient sustainable control method against the pest. With this we could develop a strategy of potato production with the intention of diminishing or even preventing the appearance of pest resistance to insecticides. The activity of these biological agents was assessed at three different temperatures (15, 20, and 25 °C) and three concentrations (200, 1000, and 2000 infective juveniles per individual). Mortality of three stages (young and old larvae and adults) was determined 2, 4, and 7 days after treatment. At 15 °C entomopathogenic nematodes showed the lowest efficacy against all insect stages. No significant differences in efficacy was determined at 20 and 25 °C as all nematodes caused prompt death of all stages. At all temperatures young larvae were most susceptible. However, when controlling overwintered adults for the purpose of preventing the mass appearance of Colorado potato beetle, we recommend an application of higher concentrations of S. feltiae suspension.

Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae)

Biotechnical Faculty, Dept. of Agronomy, Chair of Phytomedicine, Agricultural Engineering, Crop Production, Grassland and Pasture Management, University of Ljubljana, Jamnikarjeva 101, SI-1111, Ljubljana, Slovenia

Natural History Museum, Njegoševa 51, 11000, Belgrade, Serbia

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Four entomopathogenic nematode species (Steinernema feltiae, S. carpocapsae, Heterorhabditis bacteriophora, and H. megidis) were tested in a laboratory...

Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae)

Published Online: Apr 25, 2009

Page range: 14 - 20

DOI: https://doi.org/10.2478/s11687-009-0003-1

Keywords
biological control, efficacy, laboratory conditions, Leptinotarsa decemlineata

© 2009 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae)

Published Online: Apr 25, 2009

Page range: 14 - 20

DOI: https://doi.org/10.2478/s11687-009-0003-1

Keywordsbiological control, efficacy, laboratory conditions, Leptinotarsa decemlineata

© 2009 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
biological control, efficacy, laboratory conditions, Leptinotarsa decemlineata