<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Butanol is a promising alternative to fossil-derived fuels. <italic>Clostridium</italic> genus bacteria are known for their ability to produce butanol as one of the metabolites, however, at the moment this solution is not economically viable. To solve it, the process of butanol production should be optimized. While ABE fermentation has been extensively studied, information about the optimal growth conditions for specific microorganisms often differs from one study to another. Therefore, this study aims to search for optimal growth conditions in sealed serum bottle tests for three widely used strains in ABE fermentation. In this study effects of temperature, pH, and agitation were tested on <italic>Clostridium acetobutylicum</italic>, <italic>Clostridium beijerinckii</italic>, and <italic>Clostridium saccharoperbutylacetonicum</italic>. The optimal temperature for <italic>C. beijerinckii</italic> growth and butanol production was 32 °C, the optimal agitation speed for growth was 0 rpm, but for butanol production, it was 200 rpm. For <italic>C. saccharoperbutylacetonicum</italic> growth and butanol production pH 7.5, 30 °C temperature and an agitation rate of 100 rpm were optimal, however, this effect was slight. For <italic>C. acetobutylicum</italic> cultivation optimal temperature, pH, and agitation rate were respectively 37 °C, 6.5, and 200 rpm.</p>
</abstract>

Butanol is a promising alternative to fossil-derived fuels. Clostridium genus bacteria are known for their ability to produce butanol as one of the metabolites, however, at the moment this solution is not economically viable. To solve it, the process of butanol production should be optimized. While ABE fermentation has been extensively studied, information about the optimal growth conditions for specific microorganisms often differs from one study to another. Therefore, this study aims to search for optimal growth conditions in sealed serum bottle tests for three widely used strains in ABE fermentation. In this study effects of temperature, pH, and agitation were tested on Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum. The optimal temperature for C. beijerinckii growth and butanol production was 32 °C, the optimal agitation speed for growth was 0 rpm, but for butanol production, it was 200 rpm. For C. saccharoperbutylacetonicum growth and butanol production pH 7.5, 30 °C temperature and an agitation rate of 100 rpm were optimal, however, this effect was slight. For C. acetobutylicum cultivation optimal temperature, pH, and agitation rate were respectively 37 °C, 6.5, and 200 rpm.

Effects of Temperature, pH, and Agitation on Growth and Butanol Production of <italic>Clostridium acetobutylicum</italic>, <italic>Clostridium beijerinckii</italic>, and <italic>Clostridium saccharoperbutylacetonicum</italic>

Environmental and Climate Technologies

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of Temperature, pH, and Agitation on Growth and Butanol Production of , , and 

Butanol is a promising alternative to fossil-derived fuels. Clostridium genus bacteria are known for their ability to produce butanol as one of the...

Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum

Published Online: Feb 19, 2024

Page range: 71 - 83

Received: Jul 16, 2023

Accepted: Nov 06, 2023

DOI: https://doi.org/10.2478/rtuect-2024-0007

Keywords
ABE fermentation, anaerobic fermentation, biobutanol, butanol, Clostridium, external fermentation factors, IBE fermentation

© 2024 Linda Feldmane et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum

Published Online: Feb 19, 2024

Page range: 71 - 83

Received: Jul 16, 2023

Accepted: Nov 06, 2023

DOI: https://doi.org/10.2478/rtuect-2024-0007

KeywordsABE fermentation, anaerobic fermentation, biobutanol, butanol, Clostridium, external fermentation factors, IBE fermentation

© 2024 Linda Feldmane et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
ABE fermentation, anaerobic fermentation, biobutanol, butanol, Clostridium, external fermentation factors, IBE fermentation