<abstract xmlns="http://www.w3.org/1999/xhtml">

<bold>Introduction:</bold> This study provides data on carbapenemases identified in carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolated from blood-cultures by the multiplex molecular method.
<bold>Material and method</bold>: Between October 2016 and September 2017, 47 non-duplicate Klebsiella pneumoniae (KP) were isolated from blood cultures, from hospitalized patients in the Regional Institute of Gastroenterology and Hepathology, Cluj-Napoca, Romania. Identification and antimicrobial susceptibility tests (AST) were performed by Vitek 2 Compact. The combination disks test (CDT) was used for phenotypic analysis and the LightCycler® Multiplex DNA assay was used to detect and identify the carbapenemases by the LightCycler®z 480 Instrument. The following targets were chosen: blaKPC, blaNDM, blaGES, blaIMP and blaOXA-48 genes and the Cobas® 4800 software variant 2.2.0 was used for the results interpretation.
<bold>Results</bold>: Taking into consideration the meropenem minimum inhibitory concentration (MIC), 29 KP were susceptible and 18 were not-susceptible (MIC≥0.5 µg ml-1). In the CR-KP group, the CDT identified OXA-48 (10/18) and KPC (7/18) producers. One isolate showed a noninterpretable profile. The multiplex molecular analyses confirmed the carbapenemases production as: 9 CR-KP were KPC and OXA-48 co-producers, 8 were OXA-48 and one was KPC producing strains. In CR-KP group, we found a significant correlation between the CDT and RT-PCR tests results, concerning KPC (p = 0.671). Eight phenotypic results were confirmed by molecular Light-Cycler® Multiplex DNA assay. For CR-KP co-producers (KPC and OXA-48), the CDT could indicate only one carbapenem-hydrolyzing enzyme.
<bold>Conclusion</bold>: This study highlights the CR-KP co-producers (OXA-48 and KPC). OXA-48-like is more frequently encountered in our area than other carbapenemases.
</abstract>

Introduction: This study provides data on carbapenemases identified in carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolated from blood-cultures by the multiplex molecular method.
Material and method: Between October 2016 and September 2017, 47 non-duplicate Klebsiella pneumoniae (KP) were isolated from blood cultures, from hospitalized patients in the Regional Institute of Gastroenterology and Hepathology, Cluj-Napoca, Romania. Identification and antimicrobial susceptibility tests (AST) were performed by Vitek 2 Compact. The combination disks test (CDT) was used for phenotypic analysis and the LightCycler® Multiplex DNA assay was used to detect and identify the carbapenemases by the LightCycler®z 480 Instrument. The following targets were chosen: blaKPC, blaNDM, blaGES, blaIMP and blaOXA-48 genes and the Cobas® 4800 software variant 2.2.0 was used for the results interpretation.
Results: Taking into consideration the meropenem minimum inhibitory concentration (MIC), 29 KP were susceptible and 18 were not-susceptible (MIC≥0.5 µg ml-1). In the CR-KP group, the CDT identified OXA-48 (10/18) and KPC (7/18) producers. One isolate showed a noninterpretable profile. The multiplex molecular analyses confirmed the carbapenemases production as: 9 CR-KP were KPC and OXA-48 co-producers, 8 were OXA-48 and one was KPC producing strains. In CR-KP group, we found a significant correlation between the CDT and RT-PCR tests results, concerning KPC (p = 0.671). Eight phenotypic results were confirmed by molecular Light-Cycler® Multiplex DNA assay. For CR-KP co-producers (KPC and OXA-48), the CDT could indicate only one carbapenem-hydrolyzing enzyme.
Conclusion: This study highlights the CR-KP co-producers (OXA-48 and KPC). OXA-48-like is more frequently encountered in our area than other carbapenemases.

Carbapenem resistance determinants in <italic>Klebsiella pneumoniae</italic> strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods

Microbiology Department 3-Molecular Science, Faculty of Medicine

“Iuliu Hațieganu” University of Medicine and Pharmacy

Department of Medical Informatics and Biostatistics, Faculty of Medicine

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution 4.0 International License.

Carbapenem resistance determinants in  strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods

Introduction: This study provides data on carbapenemases identified in carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolated from blood-cultures by the...

Carbapenem resistance determinants in Klebsiella pneumoniae strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods

Published Online: Jul 18, 2022

Page range: 315 - 326

Received: Jan 27, 2022

Accepted: May 21, 2022

DOI: https://doi.org/10.2478/rrlm-2022-0027

Keywords
carbapenem resistance (CR), real-time polymerase chain reaction (RT-PCR), carbapenem-resistant Klebsiella pneumoniae (CR-KP)

© 2022 Manuela Tompa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Carbapenem resistance determinants in Klebsiella pneumoniae strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods

Published Online: Jul 18, 2022

Page range: 315 - 326

Received: Jan 27, 2022

Accepted: May 21, 2022

DOI: https://doi.org/10.2478/rrlm-2022-0027

Keywordscarbapenem resistance (CR), real-time polymerase chain reaction (RT-PCR), carbapenem-resistant Klebsiella pneumoniae (CR-KP)

© 2022 Manuela Tompa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
carbapenem resistance (CR), real-time polymerase chain reaction (RT-PCR), carbapenem-resistant Klebsiella pneumoniae (CR-KP)