<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Background</bold>: Malassezia species are reported to play a role in the etiology of Psoriasis vulgaris.<bold>Objective</bold>: The aim of this study was to determine the presence, frequency, distribution, and genotyping of skin colonization of Malassezia species in Psoriasis vulgaris and to compare with healthy individuals and to investigate its relationship with the severity of the disease.<bold>Methods</bold>: Skin samples were taken from scalp, arm, body, and leg of 34 psoriasis patients (lesional/non-lesional skin) and 30 healthy volunteers. Overall, 392 skin scraping samples were taken for the isolation of Malassezia species, which were incubated on the modified-Dixon agar. Conventional culture methods were used for Malassezia species identification. In isolates, genotyping was carried out by PCR-RFLP method.<bold>Results</bold>: In the samples from psoriatic lesions, most frequently isolated Malassezia species were M.globosa and M.furfur. Similarly, the most frequently isolated species in healthy volunteers was M. globosa; followed by M.restricta and M.sympodialis. The M.furfur isolation rate in psoriatic scalp and leg lesions of the patients was significantly higher than in healthy volunteers. There was no relationship between the severity of the disease and the isolated species.<bold>Conclusion</bold>: It was found that there was a difference between patients with psoriasis and healthy controls regarding presence and frequency of Malassezia species. Therefore, our study results support the view that Malessezia species may be associated with the etiopathogenesis of psoriasis. In addition, we surmise that the treatment applications for the regulation of skin microbiota of psoriasis patients will contribute positively to the treatment of psoriasis.</abstract>

Background: Malassezia species are reported to play a role in the etiology of Psoriasis vulgaris.Objective: The aim of this study was to determine the presence, frequency, distribution, and genotyping of skin colonization of Malassezia species in Psoriasis vulgaris and to compare with healthy individuals and to investigate its relationship with the severity of the disease.Methods: Skin samples were taken from scalp, arm, body, and leg of 34 psoriasis patients (lesional/non-lesional skin) and 30 healthy volunteers. Overall, 392 skin scraping samples were taken for the isolation of Malassezia species, which were incubated on the modified-Dixon agar. Conventional culture methods were used for Malassezia species identification. In isolates, genotyping was carried out by PCR-RFLP method.Results: In the samples from psoriatic lesions, most frequently isolated Malassezia species were M.globosa and M.furfur. Similarly, the most frequently isolated species in healthy volunteers was M. globosa; followed by M.restricta and M.sympodialis. The M.furfur isolation rate in psoriatic scalp and leg lesions of the patients was significantly higher than in healthy volunteers. There was no relationship between the severity of the disease and the isolated species.Conclusion: It was found that there was a difference between patients with psoriasis and healthy controls regarding presence and frequency of Malassezia species. Therefore, our study results support the view that Malessezia species may be associated with the etiopathogenesis of psoriasis. In addition, we surmise that the treatment applications for the regulation of skin microbiota of psoriasis patients will contribute positively to the treatment of psoriasis.

Frequency, Distribution and Genotyping of <italic>Malassezia</italic> Species in Patients with <italic>Psoriasis vulgaris</italic>

Department of Dermatology, Medical Faculty

Department of Microbiology, Medical Faculty

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Frequency, Distribution and Genotyping of  Species in Patients with 

Background: Malassezia species are reported to play a role in the etiology of Psoriasis vulgaris.Objective: The aim of this study was to determine the...

Frequency, Distribution and Genotyping of Malassezia Species in Patients with Psoriasis vulgaris

Published Online: Apr 27, 2021

Page range: 217 - 227

Received: Aug 20, 2020

Accepted: Jan 14, 2021

DOI: https://doi.org/10.2478/rrlm-2021-0011

Keywords
psoriasis vulgaris, malassezia, PCR-RFLP, genotyping, modified-Dixon agar, microbiota

© 2021 Ebru Celik et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Frequency, Distribution and Genotyping of Malassezia Species in Patients with Psoriasis vulgaris

Published Online: Apr 27, 2021

Page range: 217 - 227

Received: Aug 20, 2020

Accepted: Jan 14, 2021

DOI: https://doi.org/10.2478/rrlm-2021-0011

Keywordspsoriasis vulgaris, malassezia, PCR-RFLP, genotyping, modified-Dixon agar, microbiota

© 2021 Ebru Celik et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
psoriasis vulgaris, malassezia, PCR-RFLP, genotyping, modified-Dixon agar, microbiota