<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Patients with acute leukemia develop abnormalities of haemostasis, leading not only to bleeding, but also to thrombotic complications. The pathogenesis of these complications is complex and multifactorial. Because platelets and platelet derived microparticles are key players in haemostasis and thrombosis, we presumed their roles in the prediction of bleeding and thrombotic complications. Our study groups included 24 patients with acute leukemia and 16 healthy volunteers. Platelet aggregation evaluation was performed by impedance whole blood aggregometry and the ennumeration of platelet derived microparticles was done by means of flow cytometry. Eight patints developed hemorrhagic complications associated with reduced platelet aggregation response at hospital admission. Major thrombotic events occurred in 5 patients, being preceded by increased platelet aggregation in 3 cases and high level of platelet derived microparticles in 2 cases. Our findings reveal that whole blood platelet aggregometry could be a valuable tool especially in the detection of platelet hyperreactivity and in the prediction of thrombotic events. A high level of platelet derived microparticles could also predict thrombosis. These hypotheses need further evaluation and confirmation on larger number of patients.</p></abstract>

Patients with acute leukemia develop abnormalities of haemostasis, leading not only to bleeding, but also to thrombotic complications. The pathogenesis of these complications is complex and multifactorial. Because platelets and platelet derived microparticles are key players in haemostasis and thrombosis, we presumed their roles in the prediction of bleeding and thrombotic complications. Our study groups included 24 patients with acute leukemia and 16 healthy volunteers. Platelet aggregation evaluation was performed by impedance whole blood aggregometry and the ennumeration of platelet derived microparticles was done by means of flow cytometry. Eight patints developed hemorrhagic complications associated with reduced platelet aggregation response at hospital admission. Major thrombotic events occurred in 5 patients, being preceded by increased platelet aggregation in 3 cases and high level of platelet derived microparticles in 2 cases. Our findings reveal that whole blood platelet aggregometry could be a valuable tool especially in the detection of platelet hyperreactivity and in the prediction of thrombotic events. A high level of platelet derived microparticles could also predict thrombosis. These hypotheses need further evaluation and confirmation on larger number of patients.

Evaluation of platelet aggregation and platelet derived microparticles in acute leukemia patients

“Carol Davila” University of Medicine and Pharmacy

“Coltea” Clinical Hospital, Hematology Department

“Medcenter” Central Laboratory – Center for Excellence in Laboratory Medicine and Pathology

Carol Davila” University of Medicine and Pharmacy

Revista Romana de Medicina de Laborator

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Patients with acute leukemia develop abnormalities of haemostasis, leading not only to bleeding, but also to thrombotic complications. The pathogenesis of...

Evaluation of platelet aggregation and platelet derived microparticles in acute leukemia patients

Published Online: Jun 21, 2014

Page range: 199 - 207

Received: Jan 07, 2014

Accepted: Apr 17, 2014

DOI: https://doi.org/10.2478/rrlm-2014-0015

Keywords
acute leukemia, platelet aggregation, platelet-derived-microparticles, bleeding, thrombosis

© by Andra Costache

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Evaluation of platelet aggregation and platelet derived microparticles in acute leukemia patients

Published Online: Jun 21, 2014

Page range: 199 - 207

Received: Jan 07, 2014

Accepted: Apr 17, 2014

DOI: https://doi.org/10.2478/rrlm-2014-0015

Keywordsacute leukemia, platelet aggregation, platelet-derived-microparticles, bleeding, thrombosis

© by Andra Costache

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords
acute leukemia, platelet aggregation, platelet-derived-microparticles, bleeding, thrombosis