<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Search of new approaches for harmless, non-medication treatment of body dysfunctions is still on the agenda of vet and human practitioners and researchers as well. This study presents evaluation of the effect of “Stress Relief” dietary supplement (SR) on mice behaviour under different acute short-term stress conditions and treatment duration. Five experiments were performed and in each 40 animals were randomly split into four (I–IV) groups, where I and II — non-stressed mice, III and IV — stressed animals, I and III received water with trace mineral solution (TMS), II and IV received water with SR. As stress factors, forced swimming, rodent predator odour or both together were applied. Locomotor activity under normal and stress conditions in Open Field were observed and measured by a SMART video-tracking system. Blood glucose level was measured as well. SR showed a reversal of stress-decreased locomotor activity in all stress models — distance walked increased almost twice (<italic>p</italic> &lt; 0.0001), central zone crossings and time spent in it were 2–4 times greater than in the control group (<italic>p</italic> &lt; 0.0001 and <italic>p</italic> = 0.0002, respectively), and fast movement episodes and maximal speed increased by 50–200%. In addition, complete normalisation of stress-induced elevation of blood glucose level (<italic>p</italic> &lt; 0.0001) was noted. These results demonstrate for the first time that the effect of “Stress Relief” formula (water additives–minerals processed by know-how way in Vital Force Technology using Dr. Yury Kronn method) can be observed in laboratory animals, and that the effects are significant and repeatable. SR shows fear- and stress-reducing activity, which does not sufficiently differ between 7-, 9-, 14-, 28- and 32-day treatment regimes.</p></abstract>

Search of new approaches for harmless, non-medication treatment of body dysfunctions is still on the agenda of vet and human practitioners and researchers as well. This study presents evaluation of the effect of “Stress Relief” dietary supplement (SR) on mice behaviour under different acute short-term stress conditions and treatment duration. Five experiments were performed and in each 40 animals were randomly split into four (I–IV) groups, where I and II — non-stressed mice, III and IV — stressed animals, I and III received water with trace mineral solution (TMS), II and IV received water with SR. As stress factors, forced swimming, rodent predator odour or both together were applied. Locomotor activity under normal and stress conditions in Open Field were observed and measured by a SMART video-tracking system. Blood glucose level was measured as well. SR showed a reversal of stress-decreased locomotor activity in all stress models — distance walked increased almost twice (p < 0.0001), central zone crossings and time spent in it were 2–4 times greater than in the control group (p < 0.0001 and p = 0.0002, respectively), and fast movement episodes and maximal speed increased by 50–200%. In addition, complete normalisation of stress-induced elevation of blood glucose level (p < 0.0001) was noted. These results demonstrate for the first time that the effect of “Stress Relief” formula (water additives–minerals processed by know-how way in Vital Force Technology using Dr. Yury Kronn method) can be observed in laboratory animals, and that the effects are significant and repeatable. SR shows fear- and stress-reducing activity, which does not sufficiently differ between 7-, 9-, 14-, 28- and 32-day treatment regimes.

Evaluation of “Stress Relief” Dietary Supplement on Animal Stress Level and Locomotion

Augusts Kirhenšteins Institute of Microbiology and Virology

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Search of new approaches for harmless, non-medication treatment of body dysfunctions is still on the agenda of vet and human practitioners and researchers as...

Evaluation of “Stress Relief” Dietary Supplement on Animal Stress Level and Locomotion

Published Online: Aug 22, 2018

Page range: 216 - 229

Received: Aug 30, 2017

Accepted: Feb 16, 2018

DOI: https://doi.org/10.2478/prolas-2018-0027

Keywords
“Stress Relief” dietary supplement, laboratory mice, stress, stress reduction

© 2018 Šimons Svirskis et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Evaluation of “Stress Relief” Dietary Supplement on Animal Stress Level and Locomotion

Published Online: Aug 22, 2018

Page range: 216 - 229

Received: Aug 30, 2017

Accepted: Feb 16, 2018

DOI: https://doi.org/10.2478/prolas-2018-0027

Keywords“Stress Relief” dietary supplement, laboratory mice, stress, stress reduction

© 2018 Šimons Svirskis et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
“Stress Relief” dietary supplement, laboratory mice, stress, stress reduction