<abstract xmlns="http://www.w3.org/1999/xhtml">The behaviour of light interaction with biological tissue is determined by micro-optical parameters: refractive index (<italic>n</italic>), absorption coefficient (<italic>µa</italic>), scattering coefficient (<italic>µs</italic>), and anisotropy (<italic>g</italic>). The goal of this study is to measure the optical properties of normal whole blood using He-Ne laser (wavelength 632.8 nm).The refractive index is measured using the traveller microscope. The integrating sphere method is used to measure the macro-optical parameters: total diffusive reflectance, transmittance, and collimated transmittance at wavelength 632.8 nm. The macro-optical parameters are fed to Inverse Adding Doubling (IAD) theoretical technique, to estimate the micro-optical parameters (<italic>µs</italic>, <italic>µa</italic>, <italic>g</italic>). An alternative practical method is used to measure the <italic>g</italic> value based on utilising the goniometric table. The study reveals that the refractive index (<italic>n</italic>) equals 1.395±0.0547, absorption coefficient (<italic>µa</italic>) equals 2.37 mm−1, scattering coefficient (<italic>µs</italic>) equals 55.69 mm−1, and anisotropy (g) equals 0.82.In conclusion, these findings approved, in general, the applicability of the suggested experimental set up. The set up depend on using three devices: the integrating sphere method to estimate (<italic>µs</italic>, <italic>µa</italic>, <italic>g</italic>), traveller microscope (<italic>n</italic>) and goniometer (<italic>g</italic>).</abstract>

The behaviour of light interaction with biological tissue is determined by micro-optical parameters: refractive index (n), absorption coefficient (µa), scattering coefficient (µs), and anisotropy (g). The goal of this study is to measure the optical properties of normal whole blood using He-Ne laser (wavelength 632.8 nm).The refractive index is measured using the traveller microscope. The integrating sphere method is used to measure the macro-optical parameters: total diffusive reflectance, transmittance, and collimated transmittance at wavelength 632.8 nm. The macro-optical parameters are fed to Inverse Adding Doubling (IAD) theoretical technique, to estimate the micro-optical parameters (µs, µa, g). An alternative practical method is used to measure the g value based on utilising the goniometric table. The study reveals that the refractive index (n) equals 1.395±0.0547, absorption coefficient (µa) equals 2.37 mm−1, scattering coefficient (µs) equals 55.69 mm−1, and anisotropy (g) equals 0.82.In conclusion, these findings approved, in general, the applicability of the suggested experimental set up. The set up depend on using three devices: the integrating sphere method to estimate (µs, µa, g), traveller microscope (n) and goniometer (g).

Determining the optical properties of blood using He-Ne laser and double integrating sphere set-up

Polish Journal of Medical Physics and Engineering

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The behaviour of light interaction with biological tissue is determined by micro-optical parameters: refractive index (n), absorption coefficient (µa),...

Determining the optical properties of blood using He-Ne laser and double integrating sphere set-up

Published Online: Mar 28, 2019

Page range: 1 - 5

Received: Feb 28, 2018

Accepted: Jan 05, 2019

DOI: https://doi.org/10.2478/pjmpe-2019-0001

Keywords
micro-linear optical properties, whole blood, He-Ne laser, integrating sphere, inverse adding doubling

© 2019 Habib E. Ashoor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Determining the optical properties of blood using He-Ne laser and double integrating sphere set-up

Published Online: Mar 28, 2019

Page range: 1 - 5

Received: Feb 28, 2018

Accepted: Jan 05, 2019

DOI: https://doi.org/10.2478/pjmpe-2019-0001

Keywordsmicro-linear optical properties, whole blood, He-Ne laser, integrating sphere, inverse adding doubling

© 2019 Habib E. Ashoor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
micro-linear optical properties, whole blood, He-Ne laser, integrating sphere, inverse adding doubling