<abstract xmlns="http://www.w3.org/1999/xhtml">

The goal of this study was the immobilization of different lipases as protein-coated microcrystals on K2SO4 and their uses in the synthesis of isoamyl acetate in n-hexane medium. The optimum conditions, such as lipase variety, temperature, the initial molar ratio of vinyl acetate/isoamyl alcohol, immobilized lipase amount, and reaction time were determined. The highest conversion was obtained when protein-coated microcrystals of <italic>Thermomyces lanuginosus</italic> lipase (TLL-PCMCs) was used for the synthesis of isoamyl acetate. The optimum temperature, the initial molar ratio of vinyl acetate/isoamyl alcohol, immobilized lipase amount, and reaction time were determined to be 50 °C, 3.0, 30 mg, and 360 min, respectively. Under the optimized conditions, isoamyl acetate yield was obtained as 95%. TLL-PCMCs retained 90% of their initial activity after five repeat use in the isoamyl acetate synthesis. TLL-PCMCs may be used in the preparation of industrially important aroma compounds due its ease of preparation and efficiency.
</abstract>

The goal of this study was the immobilization of different lipases as protein-coated microcrystals on K2SO4 and their uses in the synthesis of isoamyl acetate in n-hexane medium. The optimum conditions, such as lipase variety, temperature, the initial molar ratio of vinyl acetate/isoamyl alcohol, immobilized lipase amount, and reaction time were determined. The highest conversion was obtained when protein-coated microcrystals of Thermomyces lanuginosus lipase (TLL-PCMCs) was used for the synthesis of isoamyl acetate. The optimum temperature, the initial molar ratio of vinyl acetate/isoamyl alcohol, immobilized lipase amount, and reaction time were determined to be 50 °C, 3.0, 30 mg, and 360 min, respectively. Under the optimized conditions, isoamyl acetate yield was obtained as 95%. TLL-PCMCs retained 90% of their initial activity after five repeat use in the isoamyl acetate synthesis. TLL-PCMCs may be used in the preparation of industrially important aroma compounds due its ease of preparation and efficiency.

Synthesis of isoamyl acetate using protein-coated microcrystals of different lipases

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution 4.0 International License.

The goal of this study was the immobilization of different lipases as protein-coated microcrystals on K2SO4 and their uses in the synthesis of isoamyl acetate...

Synthesis of isoamyl acetate using protein-coated microcrystals of different lipases

Published Online: Jun 30, 2023

Page range: 15 - 20

DOI: https://doi.org/10.2478/pjct-2023-0012

Keywords
lipase, immobilization, protein-coated microcrystals, isoamyl acetate

© 2023 Murat Türk, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Synthesis of isoamyl acetate using protein-coated microcrystals of different lipases

Published Online: Jun 30, 2023

Page range: 15 - 20

DOI: https://doi.org/10.2478/pjct-2023-0012

Keywordslipase, immobilization, protein-coated microcrystals, isoamyl acetate

© 2023 Murat Türk, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
lipase, immobilization, protein-coated microcrystals, isoamyl acetate