<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Purpose: To investigate the relationship between the alkaline phosphatase (ALP) network of the marrow stroma and the haematopoietic regeneration after mice whole-body irradiation. Materials and methods: Three groups of mice were irradiated with a non-lethal ionising radiation dose: the fi rst one received an intraperitoneal injection of Levamisole, ALP inhibitor, 24 h before irradiation; the second one received an intraperitoneal injection of Lisinopril, haematopoiesis inhibitor, 24 h before irradiation; the third was left untreated, but irradiated. The fourth group, untreated and not irradiated, was the control. The total surface occupied by ALP positive processes, revealed by means of ALP cytochemistry in the marrow area, was evaluated semi-quantitively. Nucleated bone marrow cells were also counted. Results: ALP network began to increase 24 h after irradiation to reach a maximum after 72 h, when the bone marrow was almost become completely empty of the haematopoietic cells. This increase advances the haematopoietic recovery. This process was substantially delayed when the mice were injected with Levamisole 24 h before irradiation. On the contrary, ALP network increased strongly since the fi rst day after irradiation when the mice were injected with Lisinopril 24 h before irradiation. Conclusions: These data have indicated that the haematopoietic recovery and repopulation of the bone marrow were advanced by the ALP network recovery. </p></abstract>

Purpose: To investigate the relationship between the alkaline phosphatase (ALP) network of the marrow stroma and the haematopoietic regeneration after mice whole-body irradiation. Materials and methods: Three groups of mice were irradiated with a non-lethal ionising radiation dose: the fi rst one received an intraperitoneal injection of Levamisole, ALP inhibitor, 24 h before irradiation; the second one received an intraperitoneal injection of Lisinopril, haematopoiesis inhibitor, 24 h before irradiation; the third was left untreated, but irradiated. The fourth group, untreated and not irradiated, was the control. The total surface occupied by ALP positive processes, revealed by means of ALP cytochemistry in the marrow area, was evaluated semi-quantitively. Nucleated bone marrow cells were also counted. Results: ALP network began to increase 24 h after irradiation to reach a maximum after 72 h, when the bone marrow was almost become completely empty of the haematopoietic cells. This increase advances the haematopoietic recovery. This process was substantially delayed when the mice were injected with Levamisole 24 h before irradiation. On the contrary, ALP network increased strongly since the fi rst day after irradiation when the mice were injected with Lisinopril 24 h before irradiation. Conclusions: These data have indicated that the haematopoietic recovery and repopulation of the bone marrow were advanced by the ALP network recovery.

The relationship between the alkaline phosphatase network and the haematopoiesis in mice subjected to whole-body irradiation

Hematological Diseases Laboratory, Department of Radiation Medicine, Atomic Energy Commission of Syria (AECS), P. O. Box 6091 Damascus, Syria, Tel.: +963 11 213 2580, Fax: +963 11 611 2289

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Purpose: To investigate the relationship between the alkaline phosphatase (ALP) network of the marrow stroma and the haematopoietic regeneration after mice...

The relationship between the alkaline phosphatase network and the haematopoiesis in mice subjected to whole-body irradiation

Published Online: Sep 12, 2014

Page range: 105 - 110

Received: Feb 20, 2014

Accepted: Jun 11, 2014

DOI: https://doi.org/10.2478/nuka-2014-0012

Keywords
haematopoiesis, whole-body irradiation, alkaline phosphatase (ALP) network, Levamisole, Lisinopril

© by Khaled M. Almohamad

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The relationship between the alkaline phosphatase network and the haematopoiesis in mice subjected to whole-body irradiation

Published Online: Sep 12, 2014

Page range: 105 - 110

Received: Feb 20, 2014

Accepted: Jun 11, 2014

DOI: https://doi.org/10.2478/nuka-2014-0012

Keywordshaematopoiesis, whole-body irradiation, alkaline phosphatase (ALP) network, Levamisole, Lisinopril

© by Khaled M. Almohamad

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
haematopoiesis, whole-body irradiation, alkaline phosphatase (ALP) network, Levamisole, Lisinopril