Open Access

Molecular contamination of an animal facility during and after African swine fever virus infection

Marek Walczak
Marek Walczak
, 
Krzesimir Szymankiewicz
Krzesimir Szymankiewicz
, 
Fernando Rodriguez
Fernando Rodriguez
, 
Jordi Argilaguet
Jordi Argilaguet
, 
Boris Gavrilov
Boris Gavrilov
, 
Jacek Żmudzki
Jacek Żmudzki
, 
Maciej Kochanowski
Maciej Kochanowski
, 
Małgorzata Juszkiewicz
Małgorzata Juszkiewicz
 and 
Anna Szczotka-Bochniarz
Anna Szczotka-Bochniarz
   | Dec 19, 2023
Journal of Veterinary Research's Cover Image
About this article
Previous Article
Next Article
Cite
Published Online: Dec 19, 2023
Page range: 503 - 508
Received: Jul 18, 2023
Accepted: Nov 14, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0065
Keywords
© 2023 Marek Walczak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Survival rate and fever evolution during experimentally induced African swine fever in 10-week-old Danbred Duroc pigs. Dots indicate environmental sampling timepoints. Red dots – before disinfection; green dot – after disinfection; dashed black line – fever threshold; dpi – days post infection
Survival rate and fever evolution during experimentally induced African swine fever in 10-week-old Danbred Duroc pigs. Dots indicate environmental sampling timepoints. Red dots – before disinfection; green dot – after disinfection; dashed black line – fever threshold; dpi – days post infection

Fig. 2.

Estimated initial virus load in swab samples collected from 10-week-old Danbred Duroc pigs during experimentally induced African swine fever at 7 days post infection (dpi) and necropsy. The boxes represent the 50% between the 25 and 75% quartiles. The line inside the box indicates the median. The whiskers denote maximum and minimum values. eqTCID50 – 50% tissue culture infectious dose equivalent
Estimated initial virus load in swab samples collected from 10-week-old Danbred Duroc pigs during experimentally induced African swine fever at 7 days post infection (dpi) and necropsy. The boxes represent the 50% between the 25 and 75% quartiles. The line inside the box indicates the median. The whiskers denote maximum and minimum values. eqTCID50 – 50% tissue culture infectious dose equivalent

Fig. 3.

Initial load of African swine fever virus in different pigsty locations after experimental infection and its change over time. Statistically significant decreases of estimated initial virus load were observed at 15 dpi at the floor and drain spots – locations mechanically cleaned by water. Error bars indicate standard deviation. eqTCID50 – 50% tissue culture infectious dose equivalent; HEPA – high-efficiency particulate-absorbing; * – statistically significant (P-value 0.002 (floor), 0.0393 (drain))
Initial load of African swine fever virus in different pigsty locations after experimental infection and its change over time. Statistically significant decreases of estimated initial virus load were observed at 15 dpi at the floor and drain spots – locations mechanically cleaned by water. Error bars indicate standard deviation. eqTCID50 – 50% tissue culture infectious dose equivalent; HEPA – high-efficiency particulate-absorbing; * – statistically significant (P-value 0.002 (floor), 0.0393 (drain))

Numbers of positive qPCR-samples in tested animal facility locations and their mean threshold cycle (Ct) values

Time Number of positive samples (Mean Ct (±SD))
Floor Wall Door HEPA filter cover Drain Feeder Drinker Mat
T1 14/15(35.9 (±1.6)) 3/3(33.3 (±1.9)) 0/3 2/3(37.3 (±0.4)) 2/3(28.7 (±1.4)) 3/3(34.3 (±1.9)) 3/3(33.0 (±0.8)) 2/3(38.3 (±0.6))
T2 4/15(38.2 (±0.6)) 2/3(36.7 (±2.2)) 0/3 1/3(36.7 (±0.0)) 1/3(32.4 (±0.2)) 3/3(35.9 (±1.2)) 3/3(36.7 (±1.6) 3/3(37.8 (±0.9))
T3 5/15(39.0 (±0.8)) 2/3(35.6 (±2.9)) 0/3 1/3(39.1 (±0.0)) 1/3(32.9 (±0.6)) 3/3(36.2 (±1.6)) 3/3(34.6 (±1.6)) 3/3(37.4 (±0.7))
eISSN:
2450-8608
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
Journal RSS Feed