<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein. In the dog, which represents both an important pet species and a good animal model for studying biological and pathological testicular processes, the presence of testin has never been reported.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>In the present study, the expression of testin in foetal, prepubertal, adult and aged canine testes was investigated. Testes from 5 adult and 3 aged dogs, from 2 one-month-old puppies and from 2 foetuses miscarried at the end of pregnancy were immunohistochemically examined with a commercial antibody against testin.</p>
</sec>
<sec>
<h3>Results</h3>
<p>Testin was intensely expressed in Sertoli cells in every testis examined. Spermatids were also positive for testin in mature dogs and in the testicular areas of the aged ones which were not atrophic. Weak expression of testin was also detected in all testes examined.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>The present study, the first demonstrating the presence of testin in canine testes, provides the basis for further dog–human comparative research and for studies on the role of this protein in canine physiology, reproduction and testicular pathologies.</p>
</sec>
</abstract>

Introduction
Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein. In the dog, which represents both an important pet species and a good animal model for studying biological and pathological testicular processes, the presence of testin has never been reported.


Material and Methods
In the present study, the expression of testin in foetal, prepubertal, adult and aged canine testes was investigated. Testes from 5 adult and 3 aged dogs, from 2 one-month-old puppies and from 2 foetuses miscarried at the end of pregnancy were immunohistochemically examined with a commercial antibody against testin.


Results
Testin was intensely expressed in Sertoli cells in every testis examined. Spermatids were also positive for testin in mature dogs and in the testicular areas of the aged ones which were not atrophic. Weak expression of testin was also detected in all testes examined.


Conclusion
The present study, the first demonstrating the presence of testin in canine testes, provides the basis for further dog–human comparative research and for studies on the role of this protein in canine physiology, reproduction and testicular pathologies.

Study on the expression of testin in the testes of dogs

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Study on the expression of testin in the testes of dogs"}

Introduction
Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its...

Study on the expression of testin in the testes of dogs

Published Online: Dec 19, 2023

Page range: 627 - 633

Received: May 17, 2023

Accepted: Oct 03, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0055

Keywords
testin, testes, dog, immunohistochemistry, TES gene

© 2023 Rafał Ciaputa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Study on the expression of testin in the testes of dogs

Published Online: Dec 19, 2023

Page range: 627 - 633

Received: May 17, 2023

Accepted: Oct 03, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0055

Keywordstestin, testes, dog, immunohistochemistry, TES gene

© 2023 Rafał Ciaputa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
testin, testes, dog, immunohistochemistry, TES gene