<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>The development of genetic research over recent decades has enabled the discovery of new genetic markers, such as single nucleotide polymorphisms (SNPs). This, as well as the full sequencing of the dog genome, has enabled genome-wide association studies (GWAS) to be used in the search for genetic causes of canine mammary tumours (CMTs).</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>Genotypic data containing 175,000 SNPs, which had been obtained using the Illumina CanineHD BeadChip microarray technique, were available for analysis in this study. The data concerned 118 bitches, including 36 animals with CMT, representing various breeds and age groups. Statistical analysis was performed in two steps: quality control of genotyping data and genome-wide association analysis based on dominant, recessive, overdominant, codominant, and log-additive models with the single SNP effects.</p>
</sec>
<sec>
<h3>Results</h3>
<p>A total of 40 different SNPs significantly associated with CMT appearance were detected. Moreover, twelve SNPs showed statistical significance in more than one model. Of all the significant SNPs, two, namely <italic>BICF2G630136001</italic> in the overdominant model and <italic>TIGRP2P107898_rs9044787</italic> in the log-additive model, reached the 5<sup>−8</sup> significance level. The other SNPs were significant to a 1<sup>−5</sup> level.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>In the group of SNPs indicated as significant in the GWAS analysis, several transpired to be localised within genes that may play an important role in CMT.</p>
</sec>
</abstract>

Introduction
The development of genetic research over recent decades has enabled the discovery of new genetic markers, such as single nucleotide polymorphisms (SNPs). This, as well as the full sequencing of the dog genome, has enabled genome-wide association studies (GWAS) to be used in the search for genetic causes of canine mammary tumours (CMTs).


Material and Methods
Genotypic data containing 175,000 SNPs, which had been obtained using the Illumina CanineHD BeadChip microarray technique, were available for analysis in this study. The data concerned 118 bitches, including 36 animals with CMT, representing various breeds and age groups. Statistical analysis was performed in two steps: quality control of genotyping data and genome-wide association analysis based on dominant, recessive, overdominant, codominant, and log-additive models with the single SNP effects.


Results
A total of 40 different SNPs significantly associated with CMT appearance were detected. Moreover, twelve SNPs showed statistical significance in more than one model. Of all the significant SNPs, two, namely BICF2G630136001 in the overdominant model and TIGRP2P107898_rs9044787 in the log-additive model, reached the 5−8 significance level. The other SNPs were significant to a 1−5 level.


Conclusion
In the group of SNPs indicated as significant in the GWAS analysis, several transpired to be localised within genes that may play an important role in CMT.

Identification of SNP markers for canine mammary gland tumours in females based on a genome-wide association study – preliminary results

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
The development of genetic research over recent decades has enabled the discovery of new genetic markers, such as single nucleotide polymorphisms...

Identification of SNP markers for canine mammary gland tumours in females based on a genome-wide association study – preliminary results

Published Online: Sep 20, 2023

Page range: 427 - 436

Received: Jan 21, 2023

Accepted: Jun 28, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0040

Keywords
dogs, canine mammary tumours, genetic marker, genome-wide association studies, SNP

© 2023 Anna Mucha et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of SNP markers for canine mammary gland tumours in females based on a genome-wide association study – preliminary results

Published Online: Sep 20, 2023

Page range: 427 - 436

Received: Jan 21, 2023

Accepted: Jun 28, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0040

Keywordsdogs, canine mammary tumours, genetic marker, genome-wide association studies, SNP

© 2023 Anna Mucha et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dogs, canine mammary tumours, genetic marker, genome-wide association studies, SNP