group | fa/fa H2O | fa/fa 100 | fa/fa 200 | fa/+ 100 | fa/+ 200 |
---|---|---|---|---|---|
n | 6 | 6 | 6 | 6 | 6 |
Mean | 407.14 | 382.08 | 392.75 | 358.97 | 337.69 |
Standard deviation | 32.68 | 35.48 | 36.28 | 12.90 | 29.34 |
Index | fa/fa H2O | fa/fa 100 | fa/fa 200 | fa/+ 100 | fa/+ 200 | |||||
---|---|---|---|---|---|---|---|---|---|---|
t1 | t2 | t1 | t2 | t1 | t2 | t1 | t2 | t1 | t2 | |
17.50 ± 5.90 | 59.40 ± 11.30 | 21.40 ± 6.40 | 63.00 ± 12.80 | 21.10 ± 8.50 | 57.80 ± 8.50 | 7.60 ± 0.60 | 23.00 ± 3.70 | 7.70 ± 1.20 | 22.90 ± 2.80 | |
4.30 ± 0.30 | 5.10 ± 0.70 | 4.30 ± 0.20 | 4.80 ± 0.50 | 4.10 ± 0.10 | 5.10 ± 1.00 | 3.40 ± 0.20 | 2.20 ± 0.30 | 3.60 ± 0.10 | 2.40 ± 0.10 | |
2.50 ± 0.10 | 2.90 ± 0.40 | 2.40 ± 0.20 | 3.00 ± 0.40 | 2.40 ± 0.20 | 3.20 ± 0.60 | 1.70 ± 0.10 | 1.30 ± 0.20 | 1.80 ± 0.10 | 1.40 ± 0.10 | |
2.20 ± 0.10 | 2.80 ± 0.20 | 1.90 ± 0.10 | 2.80 ± 0.20 | 1.90 ± 0.20 | 2.80 ± 0.40 | 1.20 ± 0.20 | 1.20 ± 0.20 | 1.20 ± 0.10 | 1.10 ± 0.10 | |
11.50 ± 2.70 | 5.60 ± 5.60 | 12.70 ± 3.40 | 5.70 ± 2.90 | 12.50 ± 2.80 | 5.90 ± 1.30 | 1.30 ± 0.30 | 1.20 ± 0.60 | 1.30 ± 0.30 | 0.90 ± 0.60 |
Index | fa/fa H2O | fa/fa 100 | fa/fa 200 | fa/+ 100 | fa/+ 200 | |||||
---|---|---|---|---|---|---|---|---|---|---|
t1 | t2 | t1 | t2 | t1 | t2 | t1 | t2 | t1 | t2 | |
10.40 ± 1.63 | 7.05 ± 1.96 | 10.50 ± 1.26 | 6.02 ± 2.33 | 11.26 ± 2.56 | 6.40 ± 1.29 | 8.52 ± 0.86 | 5.00 ± 1.41 | 9.38 ± 0.99 | 3.73 ± 1.25 | |
7.49 ± 0.21 | 8.36 ± 0.69 | 7.63 ± 0.23 | 8.59 ± 0.44 | 7.60 ± 0.13 | 8.19 ± 0.53 | 7.53 ± 0.64 | 8.07 ± 0.54 | 7.69 ± 0.24 | 7.82 ± 0.63 | |
16.13 ± 0.37 | 14.53 ± 0.99 | 16.10 ± 0.37 | 14.80 ± 0.65 | 15.90 ± 0.34 | 14.32 ± 0.73 | 15.70 ± 0.73 | 13.88 ± 0.88 | 16.05 ± 0.42 | 13.90 ± 0.35 | |
43.47 ± 1.25 | 43.23 ± 3.31 | 39.18 ± 12.49 | 44.52 ± 2.32 | 44.12 ± 0.92 | 42.22 ± 2.66 | 41.88 ± 3.76 | 41.86 ± 2.61 | 42.75 ± 1.48 | 39.75 ± 3.18 | |
1,069.33 ± 207.16 | 748.00 ± 76.58 | 1033.83 ± 74.09 | 658.67 ± 67.82 | 1078.80 ± 76.59 | 718.20 ± 52.11 | 905.80 ± 53.27 | 656.60 ± 244.39 | 911.67 ± 60.07 | 738.00 ± 39.33 | |
58.00 ± 1.10 | 51.83 ± 0.75 | 57.67 ± 0.52 | 51.83 ± 0.41 | 58.00 ± 1.00 | 51.60 ± 0.55 | 55.60 ± 1.14 | 51.80 ± 0.84 | 55.50 ± 0.55 | 51.00 ± 0.00 | |
21.55 ± 0.58 | 17.43 ± 0.63 | 21.15 ± 0.22 | 17.25 ± 0.29 | 20.88 ± 0.40 | 17.50 ± 0.42 | 20.90 ± 1.07 | 17.18 ± 0.26 | 20.90 ± 0.19 | 17.87 ± 1.11 | |
37.15 ± 0.69 | 33.65 ± 1.13 | 36.50 ± 0.49 | 33.30 ± 0.62 | 36.02 ± 0.29 | 33.92 ± 0.73 | 37.62 ± 2.00 | 33.20 ± 0.17 | 37.58 ± 0.42 | 35.08 ± 2.23 | |
2.17 ± 0.87 | 1.85 ± 1.56 | 2.72 ± 0.31 | 1.15 ± 0.82 | 3.60 ± 0.67 | 0.92 ± 0.33 | 2.34 ± 0.61 | 1.12 ± 1.11 | 1.48 ± 0.69 | 0.53 ± 0.30 | |
13.33 ± 6.92 | 25.50 ± 4.76 | 19.33 ± 8.04 | 21.50 ± 4.59 | 20.40 ± 5.08 | 33.20 ± 5.72 | 16.25 ± 7.37 | 20.75 ± 3.95 | 12.83 ± 5.53 | 18.67 ± 4.50 | |
81.17 ± 5.00 | 71.50 ± 5.68 | 76.83 ± 10.26 | 74.83 ± 6.11 | 78.40 ± 5.13 | 61.80 ± 4.76 | 81.00 ± 8.68 | 75.75 ± 5.68 | 84.67 ± 4.63 | 79.50 ± 4.46 | |
2.50 ± 3.51 | 0.83 ± 0.98 | 1.00 ± 0.89 | 0.33 ± 0.52 | 0.20 ± 0.45 | 2.00 ± 1.58 | 0.50 ± 1.00 | 0.25 ± 0.50 | 1.83 ± 1.33 | 0.17 ± 0.41 | |
1.83 ± 0.75 | 0.67 ± 0.52 | 1.50 ± 1.38 | 2.33 ± 2.42 | 0.60 ± 0.55 | 1.60 ± 1.34 | 2.00 ± 1.15 | 2.50 ± 1.29 | 0.33 ± 0.52 | 1.33 ± 1.03 |
Rt (min) | MS (M-H)− |
MS/MS (M-H)− |
Name of compounds | Polyphenol content |
---|---|---|---|---|
1.67 | 331 | 271/169 | Galloyl-glucose | 2.00 ± 0.03 |
1.73 | 781 | 721/601 | Punicalin α/A | 3.11 ± 0.06 |
2.02 | 1083 | 611/331/146 | HHDP-galloyl-hexoside (punicalagin) | 4.20 ± 0.09 |
2.12 | 1083 | 781/622/301 | Punicalagin isomer | 14.82 ± 1.04 |
2.33 | 933 | 631/450/301 | Ellagitannin | 4.71 ± 0.40 |
2.87 | 1083 | 781/301 | HHDP-gallagyl-hexoside (punicalagin) | 93.91 ± 2.05 |
3.12 | 1085 | 907/783/301 | Ellagic acid derivative | 2.49 ± 0.53 |
3.69 | 1083 | 781/301 | HHDP-gallagyl-hexoside (punicalagin) | 157.00 ± 2.65 |
3.89 | 799 | 301 | Granatin A | 4.74 ± 0.32 |
5.08 | 783 | 481/301 | Ellagitannin | 25.86 ± 1.53 |
6.20 | 1085 | 933/301 | Digalloyl-gallagyl-hexoside | 10.37 ± 0.65 |
6.25 | 783 | 481/301 | Ellagitannin | 13.51 ± 0.99 |
6.38 | 463 | 301 | Ellagic acid-hexoside | 33.63 ± 1.23 |
6.89 | 951 | 907/635/301 | Galloyl-HHDP-DHHDP-hex (granatin B) | 2.68 ± 0.11 |
Total (mg/g dry weight) | 373.05 |
Index | fa/fa H2O | fa/fa 100 | fa/fa 200 | fa/+ 100 | fa/+ 200 | |||||
---|---|---|---|---|---|---|---|---|---|---|
t1 | t2 | t1 | t2 | t1 | t2 | t1 | t2 | t1 | t2 | |
289.00 ± 28.08 | 234.33 ± 37.03 | 265.00 ± 21.02 | 232.25 ± 45.85 | 245.17 ± 21.34 | 226.83 ± 32.62 | 264.60 ± 20.40 | 230.60 ± 46.55 | 275.20 ± 11.82 | 245.80 ± 31.40 | |
2.52 ± 0.54 | 2.65 ± 1.03 | 3.35 ± 1.61 | 2.65 ± 1.03 | 3.35 ± 1.23 | 2.90 ± 1.01 | 3.36 ± 0.69 | 2.36 ± 1.19 | 2.80 ± 0.70 | 2.28 ± 0.42 | |
6.48 ± 0.51 | 6.08 ± 1.15 | 6.65 ± 0.83 | 5.82 ± 0.74 | 6.87 ± 1.25 | 6.52 ± 0.95 | 6.08 ± 1.13 | 5.56 ± 1.39 | 5.84 ± 0.77 | 5.68 ± 0.69 | |
2.93 ± 0.32 | 3.00 ± 0.39 | 2.72 ± 0.43 | 3.00 ± 0.55 | 2.62 ± 0.39 | 2.77 ± 0.67 | 2.68 ± 0.49 | 3.32 ± 0.72 | 2.38 ± 0.49 | 3.18 ± 0.72 | |
1.80 ± 0.39 | 1.95 ± 0.42 | 1.78 ± 0.39 | 2.22 ± 0.38 | 1.88 ± 0.28 | 2.03 ± 0.43 | 1.98 ± 0.56 | 1.86 ± 0.19 | 1.62 ± 0.30 | 1.94 ± 0.42 | |
3.32 ± 0.38 | 3.15 ± 0.62 | 3.10 ± 0.55 | 3.67 ± 0.71 | 3.68 ± 0.55 | 3.22 ± 0.41 | 3.04 ± 1.10 | 3.24 ± 0.58 | 3.30 ± 0.71 | 3.38 ± 0.36 | |
1.77 ± 0.16 | 1.77 ± 0.29 | 1.73 ± 0.13 | 2.00 ± 0.27 | 1.92 ± 0.23 | 1.80 ± 0.43 | 1.96 ± 0.55 | 1.62 ± 0.46 | 1.78 ± 0.19 | 1.86 ± 0.30 | |
0.63 ± 0.15 | 0.55 ± 0.26 | 0.68 ± 0.22 | 0.48 ± 0.22 | 0.80 ± 0.38 | 0.65 ± 0.26 | 0.54 ± 0.26 | 0.44 ± 0.37 | 0.50 ± 0.16 | 0.44 ± 0.15 | |
0.05 ± 0.05 | 0.07 ± 0.05 | 0.15 ± 0.17 | 0.05 ± 0.10 | 0.13 ± 0.12 | 0.08 ± 0.08 | 0.10 ± 0.07 | 0.04 ± 0.09 | 0.06 ± 0.09 | 0.02 ± 0.04 | |
0.60 ± 0.14 | 0.50 ± 0.24 | 0.58 ± 0.10 | 0.40 ± 0.22 | 0.65 ± 0.31 | 0.57 ± 0.20 | 0.44 ± 0.21 | 0.40 ± 0.28 | 0.40 ± 0.16 | 0.38 ± 0.13 | |
0.17 ± 0.04 | 0.12 ± 0.07 | 0.15 ± 0.02 | 0.09 ± 0.04 | 0.16 ± 0.07 | 0.14 ± 0.06 | 0.12 ± 0.06 | 0.09 ± 0.04 | 0.11 ± 0.04 | 0.10 ± 0.03 | |
92.85 ± 3.36 | 89.83 ± 6.38 | 83.50 ± 14.01 | 84.12 ± 18.64 | 85.80 ± 8.92 | 89.55 ± 5.71 | 80.68 ± 7.18 | 90.22 ± 6.40 | 87.00 ± 7.26 | 92.64 ± 1.78 | |
61.25 ± 6.78 | 57.72 ± 12.38 | 51.52 ± 18.55 | 53.75 ± 21.09 | 52.07 ± 12.09 | 56.62 ± 10.47 | 44.80 ± 8.12 | 58.58 ± 13.68 | 52.18 ± 8.29 | 59.94 ± 3.22 | |
25.40 ± 3.67 | 22.05 ± 3.24 | 21.50 ± 7.14 | 18.10 ± 12.31 | 20.95 ± 4.67 | 25.25 ± 3.25 | 18.84 ± 3.43 | 15.22 ± 3.37 | 19.92 ± 1.76 | 19.80 ± 7.22 | |
4.22 ± 0.71 | 3.57 ± 0.47 | 4.34 ± 1.08 | 3.82 ± 0.36 | 4.87 ± 0.53 | 3.83 ± 0.69 | 4.30 ± 0.58 | 4.15 ± 0.42 | 4.58 ± 0.42 | 3.76 ± 0.29 | |
3.37 ± 0.38 | 3.23 ± 0.35 | 3.34 ± 0.73 | 3.44 ± 0.25 | 3.85 ± 0.23 | 3.12 ± 0.51 | 3.28 ± 0.39 | 3.65 ± 0.83 | 3.44 ± 0.22 | 3.12 ± 0.33 | |
1.25 ± 0.19 | 1.10 ± 0.08 | 1.30 ± 0.11 | 1.12 ± 0.13 | 1.26 ± 0.11 | 1.24 ± 0.17 | 1.31 ± 0.08 | 1.17 ± 0.18 | 1.34 ± 0.18 | 1.21 ± 0.13 |