<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0069_s_005_w2aab3b7c32b1b6b1aab1c15b1Aa"><h3>Introduction</h3><p>Three strains of the FMD virus (A, O, and SAT 2) were recognised as causes of the FMD circulating in Egypt. The aims of this study were to trace the FMDV isolates from outbreaks in Egypt to understand their epidemiology and evolution and to understand the situation of the vaccine strains compared with the circulating serotypes.</p></sec><sec id="j_jvetres-2020-0069_s_006_w2aab3b7c32b1b6b1aab1c15b2Aa"><h3>Material and Methods</h3><p>A meta-analysis was carried out by using the data available for FMD outbreaks in Egypt from GenBank and the World Reference Laboratory for Foot-and-Mouth Disease (WRLFMD); a comparison was done with both data sets for the three serotypes. MEGA-X was used for the evolution analysis, through constructions of phylogenetic trees for all sequences recorded in GenBank for each serotype in different Egyptian outbreaks in different years and also within the same year. Additionally, nucleotide substitution rate, molecular clock, and mean evolutionary rates were estimated for the three serotypes to understand and compare their evolution.</p></sec><sec id="j_jvetres-2020-0069_s_007_w2aab3b7c32b1b6b1aab1c15b3Aa"><h3>Results</h3><p>Absence of some records of certain serotype outbreaks from the WRLFMD database was noted as were subsequent missing appropriate vaccine programmes. Genetic variation was recorded among the virus isolates within the same years and also the vaccine strain was associated with up to 26 amino acid substitutions. The evolution rate of the SAT2 strain was the highest of the circulating strains. SAT2 had high amino acid substitution per year at an important immunogenic site (130–170), serotype A had less, and serotype O the least.</p></sec><sec id="j_jvetres-2020-0069_s_008_w2aab3b7c32b1b6b1aab1c15b4Aa"><h3>Conclusion</h3><p>The need for different strategies for vaccine serotype selection is indicated.</p></sec></abstract>

IntroductionThree strains of the FMD virus (A, O, and SAT 2) were recognised as causes of the FMD circulating in Egypt. The aims of this study were to trace the FMDV isolates from outbreaks in Egypt to understand their epidemiology and evolution and to understand the situation of the vaccine strains compared with the circulating serotypes.Material and MethodsA meta-analysis was carried out by using the data available for FMD outbreaks in Egypt from GenBank and the World Reference Laboratory for Foot-and-Mouth Disease (WRLFMD); a comparison was done with both data sets for the three serotypes. MEGA-X was used for the evolution analysis, through constructions of phylogenetic trees for all sequences recorded in GenBank for each serotype in different Egyptian outbreaks in different years and also within the same year. Additionally, nucleotide substitution rate, molecular clock, and mean evolutionary rates were estimated for the three serotypes to understand and compare their evolution.ResultsAbsence of some records of certain serotype outbreaks from the WRLFMD database was noted as were subsequent missing appropriate vaccine programmes. Genetic variation was recorded among the virus isolates within the same years and also the vaccine strain was associated with up to 26 amino acid substitutions. The evolution rate of the SAT2 strain was the highest of the circulating strains. SAT2 had high amino acid substitution per year at an important immunogenic site (130–170), serotype A had less, and serotype O the least.ConclusionThe need for different strategies for vaccine serotype selection is indicated.

Meta-analysis of genetic diversity of the VP1 gene among the circulating O, A, and SAT2 serotypes and vaccine strains of FMD virus in Egypt

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionThree strains of the FMD virus (A, O, and SAT 2) were recognised as causes of the FMD circulating in Egypt. The aims of this study were to trace...

Meta-analysis of genetic diversity of the VP1 gene among the circulating O, A, and SAT2 serotypes and vaccine strains of FMD virus in Egypt

Published Online: Nov 06, 2020

Page range: 487 - 493

Received: Apr 08, 2020

Accepted: Oct 06, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0069

Keywords
foot-and-mouth, disease, serotypes A, O, and SAT2, vaccine, VP1

© 2020 A.F. El Nahas et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Meta-analysis of genetic diversity of the VP1 gene among the circulating O, A, and SAT2 serotypes and vaccine strains of FMD virus in Egypt

Published Online: Nov 06, 2020

Page range: 487 - 493

Received: Apr 08, 2020

Accepted: Oct 06, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0069

Keywordsfoot-and-mouth, disease, serotypes A, O, and SAT2, vaccine, VP1

© 2020 A.F. El Nahas et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
foot-and-mouth, disease, serotypes A, O, and SAT2, vaccine, VP1