Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods

Fatma Çolakoğlu; Muhammet Lütfi Selçuk

Open Access

Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods

Fatma Çolakoğlu

and

Muhammet Lütfi Selçuk

| Oct 15, 2020

Journal of Veterinary Research

Volume 64 (2020): Issue 4 (December 2020)

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Page range: 597 - 607

Received: Mar 25, 2020

Accepted: Sep 25, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0064

Keywords
chicken embryonic immune system, bursa of Fabricius, spleen, thymus, Sunset Yellow FCF

© 2020 F. Çolakoğlu et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Application of point counting grid on histological sections of the spleen

Light microscopic images of peripheral blood smears on the 21st day of incubation. Eosinophils, heterophils, and lymphocytes were seen in the peripheral blood smear from chick embryos treated with SY2,000 (A) and SY1,000 (B)

Light microscopic images of the thymus sections in different embryonic periods. A – the thymus section from a day 10 chick embryo in the control group; B – the thymus section from a day 21 chick embryo treated with SY200; C– the thymus section from a day 21 chick embryo treated with SY1,000 showing Hassall’s corpuscles; D – the thymus section from a day 21 chick embryo treated with SY2,000

The bursa of Fabricius sections in different embryonic periods. A – the bursa of Fabricius section from a day 21 chick embryo in the control group showing FAE and IFE; B – the bursa of Fabricius section from a day 10 chick embryo treated with SY200; C – the bursa of Fabricius section from a day 13 chick embryo treated with SY1,000 showing epithelial buddings; D – the bursa of Fabricius section from a day 13 chick embryo treated with SY2,000; E – normal histological image of bursa of Fabricius section from a day 16 chick embryo in the control group; F – the bursa of Fabricius section from a day 21 chick embryo treated with SY2,000

The spleen sections in different embryonic periods. A – normal histological image of a spleen section from a day 16 chick embryo in the control group; B – the spleen section from a day 21 chick embryo treated with SY200; C – the spleen section from a day 16 chick embryo treated with SY1,000 showing mononuclear cell infiltration; D – the spleen section from a day 21 chick embryo treated with SY2,000

The proportions of peripheral blood leukocyte for the sampled days of incubation (%) ±SE

Day incubation of	Group	Heterophil	Eosinophil	Lymphocyte	Monocyte	Basophil
13^th	C	+	+	+++	-	-

	SY₂₀₀	+	+	+++	-	-

	SY_1,000	++	++	+++	-	-

	SY_2,000	++	++	+++	-	-

16^th	C	+++	+	++	-	-

	SY₂₀₀	+++	+	++	-	-

		SY_1,000	+	+++	++	-	-

		SY_2,000	+	+++	++	-	-

21^st	C	58.83 ± 2.68 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	1.67 ± 0.21 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	37.83 ± 2.74 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	1.00 ± 0.26 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	0.50 ± 0.22

	SY₂₀₀	54.00 ± 3.22 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	17.67 ± 1.28 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	26.50 ± 2.72 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	0.67 ± 0.33 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	1.66 ± 0.33

	SY_1,000	24.00 ± 1.61 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	43.00 ± 3.62 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	30.50 ± 2.06 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	0.20 ± 0.21 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	0.83 ± 0.40

	SY_2,000	27.17 ± 4.30 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	47.33 ± 4.89 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	24.50 ± 5.23 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	0.17 ± 0.16 Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen	0.83 ± 0.40

Measurement data of structures in the spleen and bursa of Fabricius according to days of incubation (mean ± SE)

Day of incubation	Group	Spleen weight (g)	Spleen capsule thickness (μm)	Spleen volume (mL)	CE	FAE thickness (μm)	IFE thickness (μm)
10^th	C	0.001 ± 0.0	2.030 ± 0.112 Values within a column with no common superscripts are significant (P < 0.05)	0.452 ± 0.042	0.044	15.540 ± 1.233 Values within a column with no common superscripts are significant (P < 0.05)	17.642 ± 1.106 Values within a column with no common superscripts are significant (P < 0.05)

	SY₂₀₀	0.001 ± 0.0	1.694 ± 0.195 Values within a column with no common superscripts are significant (P < 0.05)	0.480 ± 0.033	0.042	11.092 ± 1.161 Values within a column with no common superscripts are significant (P < 0.05)	14.945 ± 0.424 Values within a column with no common superscripts are significant (P < 0.05)

		SY_1,000	0.002 ± 0.0	0.850 ± 0.317 Values within a column with no common superscripts are significant (P < 0.05)	0.451 ± 0.027	0.047	10.565 ± 0.366 Values within a column with no common superscripts are significant (P < 0.05)	13.882 ± 1.075 Values within a column with no common superscripts are significant (P < 0.05)

	SY_2,000	0.002 ± 0.0	0.834 ± 0.285 Values within a column with no common superscripts are significant (P < 0.05)	0.485 ± 0.028	0.046	9.962 ± 1.120 Values within a column with no common superscripts are significant (P < 0.05)	13.862 ± 0.878 Values within a column with no common superscripts are significant (P < 0.05)

13^th	C	0.050 ± 0.004 Values within a column with no common superscripts are significant (P < 0.05)	11.051 ± 1.897	2.538 ± 0.305	0.046	16.178 ± 2.113 Values within a column with no common superscripts are significant (P < 0.05)	20.750 ± 1.492 Values within a column with no common superscripts are significant (P < 0.05)

	SY₂₀₀	0.005 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	10.399 ± 1.070	1.784 ± 0.147	0.044	14.178 ± 1.662 Values within a column with no common superscripts are significant (P < 0.05)	16.882 ± 2.436 Values within a column with no common superscripts are significant (P < 0.05)

	SY_1,000	0.006 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	9.232 ± 0.884	2.386 ± 0.172	0.041	11.142 ± 1.281 Values within a column with no common superscripts are significant (P < 0.05)	15.392 ± 1.162 Values within a column with no common superscripts are significant (P < 0.05)

	SY_2,000	0.005 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	7.587 ± 0.639	2.172 ± 0.218	0.042	10.631 ± 0.414 Values within a column with no common superscripts are significant (P < 0.05)	14.355 ± 1.099 Values within a column with no common superscripts are significant (P < 0.05)

16^th	C	0.115 ± 0.011 Values within a column with no common superscripts are significant (P < 0.05)	16.412 ± 1.154	4.280 ± 0.392	0.029	16.166 ± 0.777 Values within a column with no common superscripts are significant (P < 0.05)	17.043 ± 1.187 Values within a column with no common superscripts are significant (P < 0.05)

	SY₂₀₀	0.011 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	15.892 ± 1.759	4.952 ± 0.589	0.045	14.525 ± 0.997 Values within a column with no common superscripts are significant (P < 0.05)	14.135 ± 1.305 Values within a column with no common superscripts are significant (P < 0.05)

	SY_1,000	0.009 ± 0.002 Values within a column with no common superscripts are significant (P < 0.05)	15.339 ± 1.247	4.536 ± 0.578	0.028	12.015 ± 0.879 Values within a column with no common superscripts are significant (P < 0.05)	13.562 ± 0.613 Values within a column with no common superscripts are significant (P < 0.05)

	SY_2,000	0.013 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	12.424 ± 0.822	4.473 ± 0.682	0.031	11.508 ± 0.408 Values within a column with no common superscripts are significant (P < 0.05)	12.525 ± 0.841 Values within a column with no common superscripts are significant (P < 0.05)

21^st	C	0.089 ± 0.012 Values within a column with no common superscripts are significant (P < 0.05)	20.418 ± 1.413 Values within a column with no common superscripts are significant (P < 0.05)	6.832 ± 0.491 Values within a column with no common superscripts are significant (P < 0.05)	0.019	21.025 ± 2.488 Values within a column with no common superscripts are significant (P < 0.05)	22.492 ± 2.609 Values within a column with no common superscripts are significant (P < 0.05)

	SY₂₀₀	0.007 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	16.386 ± 1.028 Values within a column with no common superscripts are significant (P < 0.05)	6.001 ± 0.378 Values within a column with no common superscripts are significant (P < 0.05)	0.021	14.983 ± 2.056 Values within a column with no common superscripts are significant (P < 0.05)	19.708 ± 0.857 Values within a column with no common superscripts are significant (P < 0.05)

	SY_1,000	0.009 ± 0.002 Values within a column with no common superscripts are significant (P < 0.05)	14.804 ± 0.807 Values within a column with no common superscripts are significant (P < 0.05)	4.576 ± 0.426 Values within a column with no common superscripts are significant (P < 0.05)	0.031	14.798 ± 1.640 Values within a column with no common superscripts are significant (P < 0.05)	19.002 ± 1.641 Values within a column with no common superscripts are significant (P < 0.05)

	SY_2,000	0.009 ± 0.001 Values within a column with no common superscripts are significant (P < 0.05)	14.168 ± 1.367 Values within a column with no common superscripts are significant (P < 0.05)	4.676 ± 0.143 Values within a column with no common superscripts are significant (P < 0.05)	0.024	12.920 ± 0.836 Values within a column with no common superscripts are significant (P < 0.05)	12.572 ± 1.197 Values within a column with no common superscripts are significant (P < 0.05)

eISSN:: 2450-8608
Language:: English

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Journal Subjects:: Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine

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Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods

Published Online: Oct 15, 2020

Page range: 597 - 607

Received: Mar 25, 2020

Accepted: Sep 25, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0064

Keywords
chicken embryonic immune system, bursa of Fabricius, spleen, thymus, Sunset Yellow FCF

© 2020 F. Çolakoğlu et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

The proportions of peripheral blood leukocyte for the sampled days of incubation (%) ±SE

Measurement data of structures in the spleen and bursa of Fabricius according to days of incubation (mean ± SE)

Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods

Published Online: Oct 15, 2020

Page range: 597 - 607

Received: Mar 25, 2020

Accepted: Sep 25, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0064

Keywordschicken embryonic immune system, bursa of Fabricius, spleen, thymus, Sunset Yellow FCF

© 2020 F. Çolakoğlu et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

The proportions of peripheral blood leukocyte for the sampled days of incubation (%) ±SE

Measurement data of structures in the spleen and bursa of Fabricius according to days of incubation (mean ± SE)

Keywords
chicken embryonic immune system, bursa of Fabricius, spleen, thymus, Sunset Yellow FCF