<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0063_s_005_w2aab3b7ab1b6b1aab1c14b1Aa"><h3>Introduction</h3><p>Clinoptilolite has antiviral, antibacterial, anti-inflammatory, antidiabetic, and anticancer properties due to its biological activities. In various cancer cell culture studies, it has been reported effective against tumour cells and gave positive results in treatment of various tumours in dogs. No study was found on the effects of the nanoparticulate form, nanoclinoptilolite, on cancer cells. The aim of this study was to determine its cytotoxic and apoptotic effects in canine osteosarcoma (OSA) cell culture.</p></sec><sec id="j_jvetres-2020-0063_s_006_w2aab3b7ab1b6b1aab1c14b2Aa"><h3>Material and Methods</h3><p>Doses at 50% inhibitory concentration were determined by measuring the dose- and duration-dependent cytotoxicity of nanoclinoptilolite on canine D-17 osteosarcoma cells by methylthiazol tetrazolium (MTT) test for 24 h, 48 h, and 72 h. Murine caspase-3 and -7 activity and expression levels of the <italic>BAX</italic> and <italic>BCL2</italic> genes were measured using RT-PCR to investigate the apoptotic effect.</p></sec><sec id="j_jvetres-2020-0063_s_007_w2aab3b7ab1b6b1aab1c14b3Aa"><h3>Results</h3><p>Nanoclinoptilolite decreased cell viability and induced caspase-3- and -7-mediated apoptosis in treated canine OSA cells. Furthermore, its application to canine OSA cells downregulated the expression of <italic>BCL2</italic> and upregulated the expression of proapoptotic <italic>BAX</italic>.</p></sec><sec id="j_jvetres-2020-0063_s_008_w2aab3b7ab1b6b1aab1c14b4Aa"><h3>Conclusion</h3><p>Clinoptilolite, which was previously demonstrated to have anticancer properties, decreased cell viability effectively and rapidly and increased the apoptotic cell ratio in a novel use in nanoparticle form, exhibiting this effect by increasing the <italic>BAX/BCL2</italic> ratio.</p></sec></abstract>

IntroductionClinoptilolite has antiviral, antibacterial, anti-inflammatory, antidiabetic, and anticancer properties due to its biological activities. In various cancer cell culture studies, it has been reported effective against tumour cells and gave positive results in treatment of various tumours in dogs. No study was found on the effects of the nanoparticulate form, nanoclinoptilolite, on cancer cells. The aim of this study was to determine its cytotoxic and apoptotic effects in canine osteosarcoma (OSA) cell culture.Material and MethodsDoses at 50% inhibitory concentration were determined by measuring the dose- and duration-dependent cytotoxicity of nanoclinoptilolite on canine D-17 osteosarcoma cells by methylthiazol tetrazolium (MTT) test for 24 h, 48 h, and 72 h. Murine caspase-3 and -7 activity and expression levels of the BAX and BCL2 genes were measured using RT-PCR to investigate the apoptotic effect.ResultsNanoclinoptilolite decreased cell viability and induced caspase-3- and -7-mediated apoptosis in treated canine OSA cells. Furthermore, its application to canine OSA cells downregulated the expression of BCL2 and upregulated the expression of proapoptotic BAX.ConclusionClinoptilolite, which was previously demonstrated to have anticancer properties, decreased cell viability effectively and rapidly and increased the apoptotic cell ratio in a novel use in nanoparticle form, exhibiting this effect by increasing the BAX/BCL2 ratio.

Cytotoxic and apoptotic effect of nanoclinoptilolite on canine osteosarcoma cell lines

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionClinoptilolite has antiviral, antibacterial, anti-inflammatory, antidiabetic, and anticancer properties due to its biological activities. In...

Cytotoxic and apoptotic effect of nanoclinoptilolite on canine osteosarcoma cell lines

Published Online: Oct 08, 2020

Page range: 589 - 596

Received: Dec 06, 2019

Accepted: Sep 23, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0063

Keywords
nanoclinoptilolite, canine osteosarcoma cell line, apoptosis, caspase-3 and -7, and expression

© 2020 P.A. Ulutaş et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cytotoxic and apoptotic effect of nanoclinoptilolite on canine osteosarcoma cell lines

Published Online: Oct 08, 2020

Page range: 589 - 596

Received: Dec 06, 2019

Accepted: Sep 23, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0063

Keywordsnanoclinoptilolite, canine osteosarcoma cell line, apoptosis, caspase-3 and -7, and expression

© 2020 P.A. Ulutaş et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
nanoclinoptilolite, canine osteosarcoma cell line, apoptosis, caspase-3 and -7, and expression