<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Introduction:</bold> Tiletamine-xylazine-tramadol (XFM) has few side effects and can provide good sedation and analgesia. Adenosine 5’-monophosphate-activated protein kinase (AMPK) can attenuate trigeminal neuralgia. The study aimed to investigate the effects of XFM and its specific antagonist on AMPK in different regions of the brain. <bold>Material and Methods:</bold> A model of XFM in the rat was established. A total of 72 Sprague Dawley (SD) rats were randomly divided into three equally sized groups: XFM anaesthesia (M group), antagonist (W group), and XFM with antagonist interactive groups (MW group). Eighteen SD rats were in the control group and were injected intraperitoneally with saline (C group). The rats were sacrificed and the cerebral cortex, cerebellum, hippocampus, thalamus, and brain stem were immediately separated, in order to detect AMPKα mRNA expression by quantitative PCR. <bold>Results:</bold> XFM was able to increase the mRNA expression of AMPKα1 and AMPKα2 in all brain regions, and the antagonist caused the opposite effect, although the effects of XFM could not be completely reversed in some areas. <bold>Conclusion:</bold> XFM can influence the expression of AMPK in the central nervous system of the rat, which can provide a reference for the future development of anaesthetics for animals.</p></abstract>

Introduction: Tiletamine-xylazine-tramadol (XFM) has few side effects and can provide good sedation and analgesia. Adenosine 5’-monophosphate-activated protein kinase (AMPK) can attenuate trigeminal neuralgia. The study aimed to investigate the effects of XFM and its specific antagonist on AMPK in different regions of the brain. Material and Methods: A model of XFM in the rat was established. A total of 72 Sprague Dawley (SD) rats were randomly divided into three equally sized groups: XFM anaesthesia (M group), antagonist (W group), and XFM with antagonist interactive groups (MW group). Eighteen SD rats were in the control group and were injected intraperitoneally with saline (C group). The rats were sacrificed and the cerebral cortex, cerebellum, hippocampus, thalamus, and brain stem were immediately separated, in order to detect AMPKα mRNA expression by quantitative PCR. Results: XFM was able to increase the mRNA expression of AMPKα1 and AMPKα2 in all brain regions, and the antagonist caused the opposite effect, although the effects of XFM could not be completely reversed in some areas. Conclusion: XFM can influence the expression of AMPK in the central nervous system of the rat, which can provide a reference for the future development of anaesthetics for animals.

Effects of tiletamine-xylazine-tramadol combination and its specific antagonist on AMPK in the brain of rats

Key Laboratory of the Provincial Education

Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Tiletamine-xylazine-tramadol (XFM) has few side effects and can provide good sedation and analgesia. Adenosine 5’-monophosphate-activated protein...

Effects of tiletamine-xylazine-tramadol combination and its specific antagonist on AMPK in the brain of rats

Published Online: Jun 20, 2019

Page range: 285 - 292

Received: Sep 24, 2018

Accepted: Apr 09, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0027

Keywords
brain, AMPK, xylazine, tramadol, tiletamine

© 2019 Ning Ma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of tiletamine-xylazine-tramadol combination and its specific antagonist on AMPK in the brain of rats

Published Online: Jun 20, 2019

Page range: 285 - 292

Received: Sep 24, 2018

Accepted: Apr 09, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0027

Keywordsbrain, AMPK, xylazine, tramadol, tiletamine

© 2019 Ning Ma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
brain, AMPK, xylazine, tramadol, tiletamine