<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The aim of the study was to evaluate the occurrence of enterococci in inflammatory secretions from mastitic bovine udders and to assess their antimicrobial resistance.<bold>Material and Methods:</bold> A total of 2,000 mastitic milk samples from cows were tested in 2014–2017. The isolation of enterococci was performed by precultivation in buffered peptone water, selective multiplication in a broth with sodium azide and cristal violet, and cultivation on Slanetz and Bartley agar. The identification of enterococci was carried out using Api rapid ID 32 strep kits. The antimicrobial susceptibility was evaluated using the MIC technique.<bold>Results:</bold> Enterococci were isolated from 426 samples (21.3%). <italic>Enterococcus faecalis</italic> was the predominant species (360 strains), followed by <italic>E. faecium</italic> (35 isolates), and small numbers of others. The highest level of resistance was observed to lincomycin, tetracycline, quinupristin/dalfopristin (Synercid), erythromycin, kanamycin, streptomycin, chloramphenicol, and tylosin. Single strains were resistant to vancomycin and ciprofloxacin. All isolates were sensitive to daptomycin. <italic>E. faecalis</italic> presented a higher level of resistance in comparison to <italic>E. faecium</italic>, except to nitrofurantoin.<bold>Conclusion:</bold> The results showed frequent occurrence of enterococci in mastitic cow’s milk and confirmed the high rate of their antimicrobial resistance.</abstract>

Introduction: The aim of the study was to evaluate the occurrence of enterococci in inflammatory secretions from mastitic bovine udders and to assess their antimicrobial resistance.Material and Methods: A total of 2,000 mastitic milk samples from cows were tested in 2014–2017. The isolation of enterococci was performed by precultivation in buffered peptone water, selective multiplication in a broth with sodium azide and cristal violet, and cultivation on Slanetz and Bartley agar. The identification of enterococci was carried out using Api rapid ID 32 strep kits. The antimicrobial susceptibility was evaluated using the MIC technique.Results: Enterococci were isolated from 426 samples (21.3%). Enterococcus faecalis was the predominant species (360 strains), followed by E. faecium (35 isolates), and small numbers of others. The highest level of resistance was observed to lincomycin, tetracycline, quinupristin/dalfopristin (Synercid), erythromycin, kanamycin, streptomycin, chloramphenicol, and tylosin. Single strains were resistant to vancomycin and ciprofloxacin. All isolates were sensitive to daptomycin. E. faecalis presented a higher level of resistance in comparison to E. faecium, except to nitrofurantoin.Conclusion: The results showed frequent occurrence of enterococci in mastitic cow’s milk and confirmed the high rate of their antimicrobial resistance.

Occurrence of enterococci in mastitic cow’s milk and their antimicrobial resistance

Department of Hygiene of Food of Animal Origin

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: The aim of the study was to evaluate the occurrence of enterococci in inflammatory secretions from mastitic bovine udders and to assess their...

Occurrence of enterococci in mastitic cow’s milk and their antimicrobial resistance

Published Online: Mar 28, 2019

Page range: 93 - 97

Received: Jul 11, 2018

Accepted: Feb 21, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0014

Keywords
cows, mastitis, spp., occurrence, antimicrobial resistance

© 2019 Hanna Różańska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Occurrence of enterococci in mastitic cow’s milk and their antimicrobial resistance

Published Online: Mar 28, 2019

Page range: 93 - 97

Received: Jul 11, 2018

Accepted: Feb 21, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0014

Keywordscows, mastitis, spp., occurrence, antimicrobial resistance

© 2019 Hanna Różańska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cows, mastitis, spp., occurrence, antimicrobial resistance