<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold><italic>Campylobacter jejuni</italic> is one of the most frequently reported causes of foodborne bacterial enteric disease worldwide. The main source of these microorganisms is contaminated food, especially of poultry origin. There are several molecular methods for differentiation of <italic>Campylobacter</italic> isolates at the subgenus level, and one of these is <italic>porA-</italic>typing based on the sequencing of the major outer-membrane protein (MOMP) encoding gene. The aim of the study was to test the molecular relationship of <italic>C. jejuni</italic> strains isolated at different points along the poultry food chain and assess the population structure of the isolates.<bold>Material and Methods:</bold> A total of 451 <italic>C. jejuni</italic> were used in the study, and a DNA fragment of 630 bp of the MOMP encoding gene was amplified and sequenced.<bold>Results:</bold> One hundred and ten sequence types were identified, with 69 (62.7%) unique to the isolates' origin and 30 not present in the database. The most prevalent nucleotide variant 1 was detected in 37 (8.2%) strains. These isolates were identified in all poultry sources tested, especially in faeces (15 isolates) but also in poultry carcasses and meat (11 isolates in each).<bold>Conclusion:</bold> The <italic>porA</italic> typing method was highly discriminative for <italic>C. jejuni</italic> of poultry origin since the Simpson's diversity index (D) achieved a value of 0.876, indicating considerable diversity in the bacterial population tested. The method may be further used for epidemiological investigation purposes.</abstract>

Introduction:Campylobacter jejuni is one of the most frequently reported causes of foodborne bacterial enteric disease worldwide. The main source of these microorganisms is contaminated food, especially of poultry origin. There are several molecular methods for differentiation of Campylobacter isolates at the subgenus level, and one of these is porA-typing based on the sequencing of the major outer-membrane protein (MOMP) encoding gene. The aim of the study was to test the molecular relationship of C. jejuni strains isolated at different points along the poultry food chain and assess the population structure of the isolates.Material and Methods: A total of 451 C. jejuni were used in the study, and a DNA fragment of 630 bp of the MOMP encoding gene was amplified and sequenced.Results: One hundred and ten sequence types were identified, with 69 (62.7%) unique to the isolates' origin and 30 not present in the database. The most prevalent nucleotide variant 1 was detected in 37 (8.2%) strains. These isolates were identified in all poultry sources tested, especially in faeces (15 isolates) but also in poultry carcasses and meat (11 isolates in each).Conclusion: The porA typing method was highly discriminative for C. jejuni of poultry origin since the Simpson's diversity index (D) achieved a value of 0.876, indicating considerable diversity in the bacterial population tested. The method may be further used for epidemiological investigation purposes.

Genetic diversity of <italic>Campylobacter jejuni</italic> isolated from the poultry food chain

Department of Hygiene of Food of Animal Origin

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity of  isolated from the poultry food chain

Introduction:Campylobacter jejuni is one of the most frequently reported causes of foodborne bacterial enteric disease worldwide. The main source of these...

Genetic diversity of Campylobacter jejuni isolated from the poultry food chain

Published Online: Mar 28, 2019

Page range: 35 - 40

Received: Aug 23, 2018

Accepted: Feb 20, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0012

Keywords
poultry, typing, genetic variation

© 2019 Kinga Wieczorek et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic diversity of Campylobacter jejuni isolated from the poultry food chain

Published Online: Mar 28, 2019

Page range: 35 - 40

Received: Aug 23, 2018

Accepted: Feb 20, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0012

Keywordspoultry, typing, genetic variation

© 2019 Kinga Wieczorek et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
poultry, typing, genetic variation