<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Nickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an element necessary for cell metabolism and is used in diet supplements and biomaterials, whence it may be released along with nickel.<bold>Material and Methods:</bold> BALB/3T3 and HepG2 cells were incubated with iron chloride or nickel chloride at concentrations ranging from 100 to 1,400 µM. The following mixtures were used: iron chloride 200 µM plus nickel chloride 1,000 µM, or iron chloride 1,000 µM plus nickel chloride 200 µM. The cell viability was determined with MTT, LHD, and NRU tests.<bold>Results:</bold> A decrease in cell viability was observed after incubating the BALB/3T3 and HepG2 cells with iron chloride or nickel chloride. A synergistic effect was observed after iron chloride 1,000 μM plus nickel chloride 200 μM treatment in all assays. Moreover, the same effect was observed in the pair iron chloride 200 μM plus nickel chloride 1,000 μM in the LDH and NRU assays.<bold>Conclusions:</bold> Iron (III) and nickel (II) decrease cell viability. Iron chloride at a concentration of 200 µM protects mitochondria from nickel chloride toxicity.</abstract>

Introduction: Nickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an element necessary for cell metabolism and is used in diet supplements and biomaterials, whence it may be released along with nickel.Material and Methods: BALB/3T3 and HepG2 cells were incubated with iron chloride or nickel chloride at concentrations ranging from 100 to 1,400 µM. The following mixtures were used: iron chloride 200 µM plus nickel chloride 1,000 µM, or iron chloride 1,000 µM plus nickel chloride 200 µM. The cell viability was determined with MTT, LHD, and NRU tests.Results: A decrease in cell viability was observed after incubating the BALB/3T3 and HepG2 cells with iron chloride or nickel chloride. A synergistic effect was observed after iron chloride 1,000 μM plus nickel chloride 200 μM treatment in all assays. Moreover, the same effect was observed in the pair iron chloride 200 μM plus nickel chloride 1,000 μM in the LDH and NRU assays.Conclusions: Iron (III) and nickel (II) decrease cell viability. Iron chloride at a concentration of 200 µM protects mitochondria from nickel chloride toxicity.

Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture

The John Paul II Catholic University of Lublin

Department of Anesthesiology and Intensive Care, Faculty of Medicine

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Nickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an...

Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture

Published Online: Dec 31, 2018

Page range: 535 - 542

Received: Jul 12, 2018

Accepted: Oct 16, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0067

Keywords
nickel (II), iron (III), cytotoxicity, interactions

© 2018 Sylwia Terpiłowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture

Published Online: Dec 31, 2018

Page range: 535 - 542

Received: Jul 12, 2018

Accepted: Oct 16, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0067

Keywordsnickel (II), iron (III), cytotoxicity, interactions

© 2018 Sylwia Terpiłowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
nickel (II), iron (III), cytotoxicity, interactions