<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0039_s_005_w2aab3b7b6b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Lyme borreliosis/Lyme disease is caused by <italic>Borrelia burgdorferi</italic> and is one of the most common vector-borne diseases transmitted by ticks.</p></sec><sec id="j_jvetres-2018-0039_s_006_w2aab3b7b6b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>A total of 136 <italic>Ixodes ricinus</italic> ticks, collected in the Ternopil (Ukraine) region, including 126 adults (70 females and 56 males), and 10 nymphs were examined. The identification of the species and their developmental form was based on morphological characteristics.</p></sec><sec id="j_jvetres-2018-0039_s_007_w2aab3b7b6b1b6b1aab1c16b3Aa"><h3>Results</h3><p>PCR with B5S-Bor and 23S-Bor primers resulted in <italic>Borrelia burgdorferi sensu lato</italic> DNA amplification among six ticks (4.4%). The detailed analysis based on the DNA sequencing showed the presence of DNA of <italic>Borrelia afzelii</italic> in four samples; the remaining two represented <italic>Borrelia burgdorferi sensu lato</italic> complex, although their genospecies were not determined. The research confirmed the dominance of <italic>Borrelia afzelii</italic> genospecies in the ticks from Ukraine.</p></sec><sec id="j_jvetres-2018-0039_s_008_w2aab3b7b6b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>It seems reasonable to undertake similar research in ticks from other regions of Ukraine. Knowledge in this field can be useful for public health and planning the prevention of tick-borne diseases.</p></sec></abstract>

IntroductionLyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks.Material and MethodsA total of 136 Ixodes ricinus ticks, collected in the Ternopil (Ukraine) region, including 126 adults (70 females and 56 males), and 10 nymphs were examined. The identification of the species and their developmental form was based on morphological characteristics.ResultsPCR with B5S-Bor and 23S-Bor primers resulted in Borrelia burgdorferi sensu lato DNA amplification among six ticks (4.4%). The detailed analysis based on the DNA sequencing showed the presence of DNA of Borrelia afzelii in four samples; the remaining two represented Borrelia burgdorferi sensu lato complex, although their genospecies were not determined. The research confirmed the dominance of Borrelia afzelii genospecies in the ticks from Ukraine.ConclusionIt seems reasonable to undertake similar research in ticks from other regions of Ukraine. Knowledge in this field can be useful for public health and planning the prevention of tick-borne diseases.

Prevalence of <italic>Borrelia burgdorferi sensu lato</italic> in ticks from the Ternopil region in Ukraine

Department of Food Engineering and Machines

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence of  in ticks from the Ternopil region in Ukraine

IntroductionLyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks.Material...

Area (Western Ukraine)		Number of Ixodes ricinus ticks
		Female	Male	Nymph	Total
Ternopil Oblast	Konuchy	17	9	0	26
	Biłokrynycia	19	6	0	25
	Skala-Podilska	4	21	4	29
	Papirnya	15	10	0	25
	Monastyryska	15	10	6	31
Total		70	56	10	136

Gender and form	Number of ticks	Number and % of infected ticks
Nymph	10	1 (10%)
Male	56	2 (3.6%)
Female	70	3 (4.3%)
Total	136	6 (4.4%)

Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine

Published Online: Oct 23, 2018

Page range: 275 - 280

Received: May 29, 2018

Accepted: Sep 20, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0039

Keywords
sensu lato, genospecies, ticks, Ukraine

© 2018 M. Weiner et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Number of the tested ticks and the place of collection

Number and percentage of ticks infected with Borrelia burgdorferi s. l.

Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine

Published Online: Oct 23, 2018

Page range: 275 - 280

Received: May 29, 2018

Accepted: Sep 20, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0039

Keywordssensu lato, genospecies, ticks, Ukraine

© 2018 M. Weiner et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Number of the tested ticks and the place of collection

Number and percentage of ticks infected with Borrelia burgdorferi s. l.

Keywords
sensu lato, genospecies, ticks, Ukraine