<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0028_s_005_w2aab3b7c11b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Pyrrolizidine alkaloids (PAs) are secondary metabolites produced by many plant species. Due to their toxicity PAs can pose a risk to human and animal health. To detect the toxic compounds in feed materials a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed.</p></sec><sec id="j_jvetres-2018-0028_s_006_w2aab3b7c11b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>PAs were extracted with sulphuric acid and purified with cation exchange cartridges. A newly developed solvent mixture consisting of ethyl acetate, methanol, acetonitrile, ammonia, and triethylamine was used to wash alkaloids from the cartridges. After evaporation the residues were reconstituted in water and methanol mixture and subjected to LC-MS analysis.</p></sec><sec id="j_jvetres-2018-0028_s_007_w2aab3b7c11b1b6b1aab1c16b3Aa"><h3>Results</h3><p>The developed method was validated according to SANTE/11945/2015 guidelines. The recovery was from 84.1% to 112.9%, the repeatability ranged from 3.0% to 13.6%, and the reproducibility was from 4.8% to 18.9%.</p></sec><sec id="j_jvetres-2018-0028_s_008_w2aab3b7c11b1b6b1aab1c16b4Aa"><h3>Conclusions</h3><p>A sensitive and selective method for determination of PAs in feed materials has been developed and validated. All evaluated validation parameters were in accordance with EU Reference Laboratories document no. SANTE/11945/2015. Almost 41% of the analysed feed samples were positive for the presence of at least one PA.</p></sec></abstract>

IntroductionPyrrolizidine alkaloids (PAs) are secondary metabolites produced by many plant species. Due to their toxicity PAs can pose a risk to human and animal health. To detect the toxic compounds in feed materials a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed.Material and MethodsPAs were extracted with sulphuric acid and purified with cation exchange cartridges. A newly developed solvent mixture consisting of ethyl acetate, methanol, acetonitrile, ammonia, and triethylamine was used to wash alkaloids from the cartridges. After evaporation the residues were reconstituted in water and methanol mixture and subjected to LC-MS analysis.ResultsThe developed method was validated according to SANTE/11945/2015 guidelines. The recovery was from 84.1% to 112.9%, the repeatability ranged from 3.0% to 13.6%, and the reproducibility was from 4.8% to 18.9%.ConclusionsA sensitive and selective method for determination of PAs in feed materials has been developed and validated. All evaluated validation parameters were in accordance with EU Reference Laboratories document no. SANTE/11945/2015. Almost 41% of the analysed feed samples were positive for the presence of at least one PA.

Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds

Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionPyrrolizidine alkaloids (PAs) are secondary metabolites produced by many plant species. Due to their toxicity PAs can pose a risk to human and...

Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds

Published Online: Jul 07, 2018

Page range: 183 - 191

Received: Feb 08, 2018

Accepted: Jun 08, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0028

Keywords
feed, pyrrolizidine alkaloids, LC-MS

© 2018 Ewelina Kowalczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds

Published Online: Jul 07, 2018

Page range: 183 - 191

Received: Feb 08, 2018

Accepted: Jun 08, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0028

Keywordsfeed, pyrrolizidine alkaloids, LC-MS

© 2018 Ewelina Kowalczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
feed, pyrrolizidine alkaloids, LC-MS