Open Access

Cloning and differential expression analyses of Cdc42 from sheep

Yong-Jie Yang

Yong-Jie Yang

  • Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
  • Department of Food Science, College of Agriculture, Yanbian UniversityYanji, China
Search for this author on
Sciendo|Google Scholar
Yang, Yong-Jie
, 
Zeng-Shan Liu

Zeng-Shan Liu

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
Search for this author on
Sciendo|Google Scholar
Liu, Zeng-Shan
, 
Shi-Ying Lu

Shi-Ying Lu

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
Search for this author on
Sciendo|Google Scholar
Lu, Shi-Ying
, 
Pan Hu

Pan Hu

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
Search for this author on
Sciendo|Google Scholar
Hu, Pan
, 
Chuang Li

Chuang Li

Department of Food Science, College of Agriculture, Yanbian UniversityYanji, China
Search for this author on
Sciendo|Google Scholar
Li, Chuang
, 
Waqas Ahmad

Waqas Ahmad

  • Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
  • Section of Epidemiology and Public Health, College of Veterinary and Animal SciencesJhang, Pakistan
Search for this author on
Sciendo|Google Scholar
Ahmad, Waqas
, 
Yan-Song Li

Yan-Song Li

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
Search for this author on
Sciendo|Google Scholar
Li, Yan-Song
, 
Yun-Ming Xu

Yun-Ming Xu

  • Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
  • Jiangsu Polytechnic College of Agriculture and ForestryJurong, China
Search for this author on
Sciendo|Google Scholar
Xu, Yun-Ming
, 
Feng Tang

Feng Tang

  • Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
  • College of Animal Husbandry and Veterinary Medicine, Liaoning Medical UniversityJinzhou, China
Search for this author on
Sciendo|Google Scholar
Tang, Feng
, 
Yu Zhou

Yu Zhou

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
Search for this author on
Sciendo|Google Scholar
Zhou, Yu
 and 
Hong-Lin Ren

Hong-Lin Ren

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin UniversityChangchun, China
Search for this author on
Sciendo|Google Scholar
Ren, Hong-Lin
  
Mar 30, 2018
Cloning and differential expression analyses of Cdc42 from sheep's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Published Online: Mar 30, 2018
Page range: 113 - 119
Received: Sep 07, 2017
Accepted: Feb 14, 2018
DOI: https://doi.org/10.2478/jvetres-2018-0016
Keywords
© 2018 Y.-J. Yang1et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Serological diagnosis of brucellosis is still a great challenge due to the infeasibility of discriminating infected animals from vaccinated ones, so it is necessary to search for diagnostic biomarkers for differential diagnosis of brucellosis.

Material and Methods

Cell division cycle 42 (Cdc42) from sheep (Ovis aries) (OaCdc42) was cloned by rapid amplification of cDNA ends (RACE), and then tissue distribution and differential expression levels of OaCdc42 mRNA between infected and vaccinated sheep were analysed by RT-qPCR.

Results

The full-length cDNA of OaCdc42 was 1,609 bp containing an open reading frame (ORF) of 576 bp. OaCdc42 mRNAs were detected in the heart, liver, spleen, lung, kidneys, rumen, small intestine, skeletal muscles, and buffy coat, and the highest expression was detected in the small intestine. Compared to the control, the levels of OaCdc42 mRNA from sheep infected with Brucella melitensis or sheep vaccinated with Brucella suis S2 was significantly different (P < 0.01) after 40 and 30 days post-inoculation, respectively. However, the expression of OaCdc42 mRNA was significantly different between vaccinated and infected sheep (P < 0.05 or P < 0.01) on days: 14, 30, and 60 post-inoculation, whereas no significant difference (P > 0.05) was noted 40 days post-inoculation. Moreover, the expression of OaCdc42 from both infected and vaccinated sheep showed irregularity.

Conclusion

OaCdc42 is not a good potential diagnostic biomarker for differential diagnosis of brucellosis in sheep.
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
Journal RSS Feed